Isolation, identification, and sequence analysis of rDNA-ITS of Pythium species sampled from rhizosphere soil of corn

Acta Prataculturae Sinica ›› 2009, Vol. 18 ›› Issue (3): 126-135.

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Isolation, identification, and sequence analysis of rDNA-ITS of
Pythium species sampled from rhizosphere soil of corn

CHAI Zhao-xiang^1,2,3,4, LI Jin-hua^1,2, LOU Bing-gan⁵, LI Wei⁶, GAN Hui-lin1,
GUO Cheng¹, ZHAO Ling¹, DONG Di¹

1.College of Grassland Sciences, Gansu Agricultural University, Lanzhou 730070, China;
2.Gansu Key
Lab of Crop improvement and Germplasm Enhancement, Lanzhou 730070, China;
3.Key Laboratory
of Grassland Ecosystem, Ministry of Education, Lanzhou 730070, China;
4.Sino-U.S. Centers for
Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
5.Institute of Biotechnology,
Zhejiang University, Hangzhou 310029, China;
6.College of Life Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2008-08-16 Online:2009-06-20 Published:2009-06-20

Abstract

Abstract: Pythium spp. were isolated from the rhizosphere soils of corn sampled from Honggu and Qilihe districts of Lanzhou using a baiting method. Twenty three isolates were obtained after subculture and purification. Based on the morphology, culture characteristics, growth rate, adaptation to temperatures and sequence analysis of rDNA-ITS, the 23 isolates were all identified as P. scleroteichum, P. ultimum or Pythium sp. This is the first report of P. scleroteichum in China and of P. ultimum from corn in Gansu. P. ultimum was the dominant population of the three species (60.9% of the isolations), while Pythium sp. (26.1%) was second, and, P. scleroteichum (13.0%) was third.

CLC Number:

S435.131

CHAI Zhao-xiang, LI Jin-hua, LOU Bing-gan, LI Wei, GAN Hui-lin1,
GUO Cheng, ZHAO Ling, DONG Di. Isolation, identification, and sequence analysis of rDNA-ITS of
Pythium species sampled from rhizosphere soil of corn[J]. Acta Prataculturae Sinica, 2009, 18(3): 126-135.

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Isolation, identification, and sequence analysis of rDNA-ITS of
Pythium species sampled from rhizosphere soil of corn

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