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Acta Prataculturae Sinica ›› 2009, Vol. 18 ›› Issue (3): 67-73.

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Genetic diversity identification based on ISSR analysis of Agrostis stolonifera
germplasm resources in Guizhou

SHANG Yi-shun1, YANG Chun-yan2, ZHONG Li1   

  1. 1.Guizhou Prataculturale Institute, Dushan 558200,China;
    2.Agricultural
    Bureau of Duyun, Duyun 558000,China
  • Received:2008-11-20 Online:2009-06-20 Published:2009-06-20

Abstract: Agrostis stolonifera is a widely grown, cool season turf grass in China. To determine genetic diversity and relationships of A. stolonifera resources, 285 plants from three wild populations and a cultivated population in Guizhou were screened by inter-simple sequence repeat(ISSR)markers. Nine of 60 primer pairs produced a total of 81 bands, of which 66 were polymorphic. Statistic analysis showed a rich genetic diversity of A. stolonifera resources in Guizhou province. The genetic differentiation coefficient (Gst) and the gene flow (Nm)among three wild populations were 0.492 0 and 0.516 4 .The genetic differentiation was considerable in the three wild populations. The genetic distance GD)of the subpopulations ranged from 0.087 7 to 0.592 6. The subpopulations could be clustered into three groups based on GD. Correlation analysis found a significant positive correlation between geographical distance and genetic distance (r=0.494), and this is persuasive evidence that there is a significant correlation between genetic component and the geographic distribution of A. stolonifera resources. Further genetic relationship analysis discovered a high genetic similarity among wild populations, ranged from 0.700 3 to 0.940 9 while the cultivated population (KROMI) had an obvious genetic differentiation from wild populations.

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