Effects of different conservation tillage on amount and distribution of soil ammonifying bacteria, nitrobacteria and nitrogen-fixing bacteria

Abstract

Abstract: Field experiments were conducted in Dingxi, in the western Loess Plateau, on the effects of tillage on soil ammonifying bacteria, nitrobacteria and nitrogen-fixing bacteria under a rotation system with spring wheat and pea. The treatments were conventional tillage and three conservation agriculture patterns: Conventional tillage (T), conventional tillage with stubble incorporation (TS), no till with no straw cover (NT), no till with straw cover (NTS) The amount of soil ammonifying bacteria, nitrobacteria and nitrogen-fixing bacteria from high to low was NTS, TS, NT, and T in the 0-5, 5-10, and 10-30 cm depths of soil under two rotation fields. The amounts of soil ammonifying bacteria in NTS at these three depths was 76.21%,146.92%,67.82% respectively, higher than that in NT. Soil nitrobacteria and nitrogen-fixing bacteria initially increased, then decreased in all treatments in spring wheat fields. However, soil nitrogen-fixing bacteria decreased with soil depth, while nitrobacteria initially increased, then decreased in pea fields. Ammonifying bacteria decreased with soil depth in NTS and TS, but initially increased, then decreased in NT and T.

CLC Number:

S154.36

ZHAO You-yi, CAI Li-qun, WANG Jing, ZHANG Ren-zhi. Effects of different conservation tillage on amount and distribution of soil ammonifying bacteria, nitrobacteria and nitrogen-fixing bacteria[J]. Acta Prataculturae Sinica, 2009, 18(4): 125-130.

References

[1] Prassd P. A comparative count of the microbiological characteristics of soils under natural forest, grassland and crop field from Eeastren India[J]. Plant and Soil, 1995, 175(1): 85-91.
[2] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.112-132.
[3] 土壤微生物研究会.土壤微生物实验法[M].北京:科学出版社,1983.62-139.
[4] 韩芳,邵玉琴,赵吉,等.皇甫川流域不同土地利用方式下的土壤微生物多样性[J].内蒙古大学学报(自然科学版),2003,(5):298-303.
[5] 姚槐应,何振立,黄昌勇.不同土地利用方式对红壤微生物多样性的影响[J].水土保持学报,2003,17(2):51-54.
[6] 樊丽琴,南志标,沈禹颖,等.保护性耕作对黄土高原豌豆田土壤微生物量碳的影响[J].草原与草坪,2005,(4):51-54.
[7] 范丙全,刘巧玲.保护性耕作与秸秆还田对土壤微生物及其溶磷特性的影响[J].生态农业学报,2005,13(3):130-132.
[8] 王启兰,王长庭,杜岩功,等. 放牧对高寒嵩草草甸土壤微生物量碳的影响及其与土壤环境的关系[J].草业学报,2008,17(2): 39-41.
[9] 郭彦军,韩建国.农牧交错带退耕还草对土壤酶活性的影响[J].草业学报,2008,17(5):16-23.
[10] 王丽宏,杨光立,曾昭海,等.稻田冬种黑麦草对饲草生产和土壤微生物效应的影响[J].草业学报,2008,17(2):157-161.
[11] 杨青华,韩锦峰.棉田不同覆盖方式对土壤微生物和酶活性的影响[J].土壤学报,2005,42(2):348-351.
[12] 陈锡时,郭树凡,汪景宽,等.地膜覆盖栽培对土壤微生物种群和生物活性的影响[J].应用生态学报,1998, 9(4): 435-439.
[13] 金钧然.不同林分下的土壤细菌区系[J].北京林业大学学报,1991,13(2):135-138.
[14] 刘世贵,葛绍荣,龙章富.川西北退化草地土壤微生物数量与区系研究[J].草业学报,1994,3(4):70-76.
[15] 姚拓,张德罡,胡自治.高寒地区燕麦根际联合固氮菌研究Ι.固氮菌分离及鉴定[J].草业学报, 2004, 13(2):106-111.
[16] 罗珠珠,黄高宝,张国盛.不同耕作方式对黄土高原旱地表容重和水分入渗的影响[J].干旱地区农业研究,2005,23(5):7-11.
[17] 李玲玲,黄高宝,张仁陟,等.免耕秸秆覆盖对旱作农田土壤水分的影响[J].水土保持学报,2005,19(5):95-96.
[18] 逄蕾,黄高宝.不同耕作方式对旱地土壤有机碳转化的影响[J].水土保持学报,2006,20(3):110-113.
[19] 黄高宝,李玲玲,张仁陟,等.免耕秸秆覆盖对旱作麦田土壤温度的影响[J].干旱地区农业研究,2006,24(5):1-4.
[20] 陈蓓,张仁陟.免耕与覆盖对土壤微生物数量及组成的影响[J].甘肃农业大学学报,2004, 39(6):634-638.
[21] 张萍,刀志灵,郭辉军.高黎贡山不同土地利用方式对土壤微生物数量和多样性的影响[J].云南植物研究,1999,(增刊):84-89.
[22] 姚拓,龙瑞军,师尚礼,等.高寒草地不同扰动生境土壤微生物氮素生理群数量特征研究[J].土壤学报,2007,44(1):122-128.