Genetic relationship analysis of Zea species using RAPD markers

Abstract

Abstract: Randomly amplified polymorphic DNA (RAPD) was used to identify the polymorphisms and relationships between 14 genotypes of maize and its wild relatives (teosinte). A total of 136 primers were screened from 340 random decamer primers, and a total of 5 303 DNA bands were amplified, of which 4 500 were polymorphic. Genetic similarities among the cultivars ranged from 0.570 to 0.809 and in wild species, from 0.767 to 0.809 in the same genotypes, and from 0.570 to 0.745 between different genotypes. A consensus tree indicated that the genus Zea can be classified into two sections: Zea and Luxuriantes. Section Zea consists of four subspecies: Z. mays ssp. mays, Z. mays ssp. mexicana, Z. mays ssp. parviglumis and Z. mays ssp. huehuetenangensis. Section Luxuriantes is composed of Z. diploperennis, Z. luxurians and Z. perennis, as well as Z. nicaraguensis. Z. nicaraguensis was genetically closer to Z. luxurians than to other teosintes. In conclusion, polymorphism information obtained using RAPDs in a set of genotypes is useful for the assessment of genetic relationships and could be useful in taxonomic classification programs.

CLC Number:

TANG Qi-lin, WANG Pei, LU Yan-li, ZHENG Ming-min, LIU Jian, RONG Ting-zhao. Genetic relationship analysis of Zea species using RAPD markers[J]. Acta Prataculturae Sinica, 2009, 18(4): 154-160.

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