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Acta Prataculturae Sinica ›› 2010, Vol. 19 ›› Issue (4): 135-141.

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Sequence analysis of the rDNA ITS region of Pennisetum species (Poaceae)

CHEN Zhi-tong1,2, HUANG Qin-lou2, PAN Wei-bin2,3, HUANG Yi-bin1,2   

  1. 1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

    2.Agricultural Ecology Research Institute, Fujian Academy of Agricultural Sciences/Fujian
    Engineering and Technology Research Center for Hilly Prataculture, Fuzhou 350013,
    China;
    3.Minxi Vocational and Technical College, Resource Engineering
    Department, Longyan 364021, China
  • Received:2009-06-22 Online:2010-08-20 Published:2010-08-20

Abstract:

The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from 15 species of Pennisetum (Poaceae) from areas such as Fujian, Jiangsu, Hainan were sequenced and the sequences submitted to the Genbank database. The phylogenetic relationship of the 15 species were studied by CLUSTAL X and MEGA software. Their length of nrDNA ITS region ranged from 573 to 586 bp. P. americanum×P. purpureum and P. americanum×P. purpureum cv. slimstem may be the same variety with different names. The phylogram tree indicated that sequence analysis of nrDNA ITS regions is a good technique for studying the genetic diversity of Pennisetum.

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