Identification of QTLs for nutrient contents of rice as forage

Abstract

Abstract: A genetic linkage map of 85 SSR markers was constructed based on an F₂ population derived from a cross between a cultivated rice “Taichung 65” (Oryza sativa) (♀) and a common wild rice (O. rufipogon) (♂). The markers in the linkage map were distributed on 12 rice chromosomes. The statistic software of Mapmaker/QTL 1.0 was used to detect QTLs for crude protein, crude fiber, ether extract, crude ash, silica, and water soluble carbohydrate contents. There were 3 QTLs for crude protein, 1 QTL for ether extract, 2 QTLs for silica, and 3 QTLs for water soluble carbohydrate. They were identified on chromosomes 1, 2, 4, 7, 8, 9, 10 and 11. Interval mapping identified 4 major QTLs and 5 minor QTLs. Major QTLs were qCEE-1 (explained 56.8% of variation) for the ether extract content, qCWSC-4 (explained 23.1% of variation) and qCWSC-7 (explained 25.0% of variation) for WSC content and qCS-9 (explained 15.9% of variation) for silica content. QTLs for crude fiber and crude ash contents were not detected.

CLC Number:

S816.11

FAN Chuan-guang, ZHANG Xiang-qian, ZHANG Jian-guo, LIAN Zi-xian. Identification of QTLs for nutrient contents of rice as forage[J]. Acta Prataculturae Sinica, 2010, 19(4): 142-148.

References

[1] 张建国, 刘向东, 曹致中, 等. 饲料稻研究现状及发展前景[J]. 草业学报, 2008, 17(5): 151-155.
[2] 江川, 王金英, 李清华. 野生稻的优异特性及其在水稻育种中的利用[J]. 福建稻麦科技, 2003, 21(3): 8-10.
[3] 杨庆文, 张万霞, 贺丹霞, 等. 中国野生稻原生境保护方法研究[J]. 植物遗传资源学报, 2003, 4(1): 63-67.
[4] Paterson A H, Lander E S, Hewitt J D, et al. Resolution of quantitative traits into mendelian factors by using a complete linkage map of restriction fragment length polymorphisms[J]. Nature, 1988, 335: 721-726.
[5] Lander E S, Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps[J]. Genetics, 1989,121: 185-189.
[6] Yano M, Harushima Y, Nagaruma Y, et al. Identification of quantitative trait loci controlling heading date in rice using a high-density linkage map[J]. Theoretical and Applied Genetics, 1997, 95: 1025-1032.
[7] Yamamoto T, Taguchi S F, Ukai Y. Mapping quantitative trait loci for days to heading, and culm, panicle internode lengths in a BC₁F₃ population using an elite rice variety, Koshihikari as the recurrent parent[J]. Breeding Science, 2001, 51: 63-71.
[8] Luo L J, Li Z K, Mei H W, et al. Overdominant epistatic loci are the primary genetic basic of inbreeding depression and heterosis in rice. Ⅱ. Grain yield components[J]. Genetics, 2001, 158: 1755-1771.
[9] Li Z F, Wan J M, Xia J F, et al. Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.)[J]. Acta Genetica Sinica, 2003, 30(3): 251-259.
[10] Tan Y F, Sun M, Xing Y Z, et al. Mapping quantitative trait loci for milling quality, protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid[J]. Theoretical and Applied Genetics, 2001, 103: 1037-1045.
[11] Yang Q H, Lu W, Hu M L, et al. QTL and epistatic interaction underlying leaf chlorophyll and H₂O₂ content variation in rice (Oryza sativa L.)[J]. Acta Genetica Sinica, 2003, 30(3): 245-250.
[12] 王晓娟, 孙月华, 杨晓莉, 等. 苜蓿遗传图谱构建及其应用[J]. 草业学报, 2008, 17(3): 119-127.
[13] 郑轶琦, 刘建秀. 草坪草分子遗传图谱的构建与应用研究进展[J]. 草业学报, 2009, 18(1): 155-162.
[14] 韩雅珊. 食品化学实验指导[M]. 北京: 北京农业大学出版社, 1996: 26-29.
[15] 张丽英. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业科技出版社, 2003: 46-75.
[16] 王爱民, 陈石燕, 沈革志,等. Ac/Ds(GUS)结构介导的水稻启动子捕获系统的建立[J]. 植物生理与分子生物学学报, 2005, 31(6): 575-580.
[17] Panaud O, Chen X, McCouch S. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSLP) in rice(Oryza sativa L.)[J]. Molecular Genetics and Genomics,1996, 252: 597-607.
[18] Lincoln S, Daly M, Lander E. Constructing genetics map with MAPMAKER/EXP 3.0[A]. Whitehead Institute Technical Report(3rd ed.)[C]. Masachussetts: Whitehead Institute, 1992.
[19] Kosambi D D. The estimation of map distances from recombination values[J]. Annals of Eugenics, 1994, 12: 172-175.
[20] Lander E S, Green P, Abrahamson J, et al. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations[J]. Genomics, 1987, 1: 174-181.
[21] Mclouch S R, Cho G, Yano M, et al. Report on QTL nomenclature[J]. Rice Genetics Newsletters, 1997, 14: 11-13.
[22] Graner A, Jahoor A, Schondelmaier J, et al. Construction of an RFLP map of barley[J]. Theoretical and Applied Genetics, 1991, 83: 250-256.
[23] Jansen R C, Vanooijen J W, Stam P, et al. Genotype-by-environment interaction in genetic mapping of multiple quantitative trait loci[J]. Theoretical and Applied Genetics, 1995, 91: 33-37.
[24] Song K, Slocum M K, Oshorn T C. Molecular marker analysis of genes controlling morphological variation in Brassica rapa (syn. Campestris)[J]. Theoretical and Applied Genetics, 1995, 90: 1-10.
[25] 林鸿宣. 应用RFLP标记定位水稻产量及其它有关性状的数量性状基因座位[D]. 北京: 中国农业科学院, 1994.