Abstract: A study of the anatomical structure of leaves from 20 populations of bermudagras growing in different environments found that there were significant differences in cuticular thickness, epidermal cells thickness, leaf epidermal cell lengths and widths, stomata size and density, leaf thickness, number and diameter of vascular bundles, and strengthening tissue thickness. The coefficients of variability were greater in stomatal density and in strengthening tissue thickness than in vascular bundles of midribs and the abaxial epidermis. Stomatal density ranged from 77.49 to 335.46 per mm², with a coefficient of variability up to 36.93%. Strengthening tissue thickness ranged from 7.50 to 45.00 μm, with a coefficient of variability up to 64.20%. Using the indexes measured, the 20 populations of bermudagrass could be divided into four categories by cluster analysis. The results of cluster analysis were identical with the population distributions of the bermudagrass.