Research progress on Oncidium in tissue culture and transgenes

Abstract

Abstract: This article reviews the main research progress on Oncidium tissue culture and genetic transformation. There are important effects of explants, medium and its supplements [including organic compounds and plant growth regulators (PGRs)], and culture methods for protocorm-like bodies (PLB) or crown bud induction in Oncidium in vitro culture. Flower-stalk, flower bud, apical bud and root-tip of field plants can all be used as explants in primary culture, but flower-stalks and flower buds are the best commonly used explants for Oncidium in vitro culture. All of the tissues or organs of test-tube plants are good explants for Oncidium in vitro culture. Oncidium is mainly cultured on solid media and 1/2MS or MS is the commonly used basic medium for in vitro culture. The types, concentrations of PGRs, and their combinations in the medium are key factors in Oncidium in vitro culture. The process of PLB formation in Oncidium is a typical somatic embryogenesis which is greatly influenced by PGRs, genotype and culture condition. Research on genetic transformation of Oncidium is in a primary stage.

CLC Number:

Q943.1

CUI Guang-rong. Research progress on Oncidium in tissue culture and transgenes[J]. Acta Prataculturae Sinica, 2010, 19(4): 220-229.

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