Genetic variation of Medicago sativa callus revealed by the ploidy analyser

Abstract

Abstract: DNA contents of callus of three genotype and eight culture time, which derived from anther culture in Medicago sativa, were detected by flow-cytometric analysis. The percentage of S stage cells and percentage of DNA content variation cells were analysed by the Data Pool Application of Cytometre software, the relationship between the callus cultivating time and the DNA content variation were analysed by the SPSS 10.0 software. One purpose of the study is to seek the relationship between DNA content variation and the culture time, and another is to provide the basic study of the law of DNA content variation in M. sativa for further research. DNA content of 24 samples were all changed and all DNA doubled. By the SPSS 10.0 software analysed, the correlativity between percentage of DNA content variation cells and cultivating time in M. sativa were found. With the increase of cultivating time, chromosome mutation were added. However, the trend was not always unchanged. The 0-12 week was the concentrated occurring stage of M. sativa callus DNA content variation, whereafter, DNA content variation become smooth with the increase of subculture times.

CLC Number:

GENG Xiao-li, WEI Zhen-wu, YAO Xi-hong. Genetic variation of Medicago sativa callus revealed by the ploidy analyser[J]. Acta Prataculturae Sinica, 2011, 20(3): 156-161.

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