Effects of anti-browning agents on the induction of sudangrass callus

Abstract

Abstract: Sudangrass can easily produce phenolic compounds when it is grown in tissue culture. The browning phenomenon is harmful to callus growth, leading to callus death. Anti-browning agents were used to study browning. Active carbon(AC), vitamin C(V_C), polyvinyl pyrrolidone(PVP), and AgNO₃ all improved the number of inductions and reduced the numbers browning. Low concentrations of sucrose (10-20 g/L) reduced the numbers browning but did not improve the induction number, while high concentrations increased both. Citric acid improved only the induction numbers and did not affect the numbers browning. When the concentration of AC was 0.15 g/L, the callus was best both in terms of induction number and anti-browning.

CLC Number:

LV Zong-you, SU Yan-jing, ZHAO Guo-qi, WU Cai-xia. Effects of anti-browning agents on the induction of sudangrass callus[J]. Acta Prataculturae Sinica, 2011, 20(3): 174-181.

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