An approach to detect the low-abundant proteins in rice leaf sheath

Abstract

Abstract: Enrichment of low-abundant proteins and their detection still remain great challenges in proteomics research. The existence of high-abundant proteins, e.g. ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) in plants, leads to this dilemma. The high-abundant proteins engage a large proportion of the whole-cell proteins and thus make the low-abundant proteins poorly detectable by 2-DE. In this report, we used a protocol for the preparation of whole-cell proteins through polyethylene glycol (PEG) precipitation, to develop an approach of identifying low-abundant proteins in rice leaf sheathes. In a comparison of the 2-DE analyses of protein samples prepared using the Mg/NP-40 method without PEG fractionation, with the 15% and 20% PEG fractionation protocol, a relatively high reproducibility was achieved using the 20% PEG fractionation protocol in terms of protein species and low-abundant proteins.

CLC Number:

WANG Ying, CUI Wei-tong, YANG Ming-feng, SHEN Shi-hua. An approach to detect the low-abundant proteins in rice leaf sheath[J]. Acta Prataculturae Sinica, 2011, 20(3): 192-197.

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