Effects of green manure application on the microbial biomass C and N contents and of the enzyme activity of tobacco-planting soil

Abstract

Abstract: Field experiments were conducted to study the effects of green manure application on microbial biomass C and N contents and of the enzyme activity of tobacco-planting soil. The application of green manure increased the soil microbial biomass C and N contents and the activities of soil urease, acid phosphatase (ACP), sucrase, catalase. The trend of increase returning 22 500 kg/ha was more greater than the others. Compared with the control at different growth stages, the soil microbial biomass C and N contents increased 66.09%-161.28% and 76.88%-257.10%, respectively. The activity of soil urease, acid phosphatase (ACP), invertase and catalase increased 31.88%-54.05%, 11.15%-17.62%, 16.05%-101.06% and 41.38%-71.43%, respectively. These results show that application of green manure promoted the activity of soil organisms, thus favouring the turnover of organic matter in the soil and the provision of nutrition to meet the needs of tobacco plants normal growth. The dynamic changes of soil microbial biomass C and N contents and of the enzyme activity within the tobacco developmental period showed that microbial biomass C and N reached a peak at the rosette stage of flue-cured tobacco. However, the peak of soil enzyme activities appeared at the busy stage, and when the crops were in bloom. The dynamic changes of microbial biomass C and N contents and of enzyme activity varied at different growth stages, suggesting that changes of soil enzyme activity and nutrient release from green manure were highly related with soil nutrient supply. The microbial biomass C and N contents and enzyme activity could be used as biological indices of soil quality. The relationship of the whole decomposition process of green manure with growth was coordinated with nutrient uptake of flue-cured tobacco. This study provides a theoretical basis to study low-carbon agriculture and build resource-saving, environment-friendly agriculture and promote the sustainable development of tobacco agriculture and the production of tobacco leaf with specific characteristics.

CLC Number:

S154.3

LI Zheng, LIU Guo-shun, JING Hai-xia, XIE Chang-sheng, XIANG Yong-guang, YANG Chao, ZHENG Wen-ran, YE Xie-feng. Effects of green manure application on the microbial biomass C and N contents and of the enzyme activity of tobacco-planting soil[J]. Acta Prataculturae Sinica, 2011, 20(3): 225-232.

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