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Acta Prataculturae Sinica ›› 2011, Vol. 20 ›› Issue (3): 91-98.

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Dynamic changes in the growth and the AsA-GSH circulation metabolism of Chinese cabbage treated with a biogas slurry of water hyacinth

XUE Yan-feng1,2,3, FENG Hui-fang1,4, SHI Zhi-qi1,2,3, YAN Shao-hua5, ZHENG Jian-chu5   

  1. 1.Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210094, China;
    2.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210094, China;
    3.Key Lab of Agro-Food Safety and Quality, Ministry of Agriculture, Nanjing 210094, China;
    4.College of Life Science, Nanjing Normal University, Nanjing 210097, China;
    5.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2010-05-04 Online:2011-03-25 Published:2011-06-20

Abstract: Dynamic changes in the growth and the AsA-GSH circulation metabolism of Chinese cabbage treated with different contents of biogas slurry of water hyacinth were studied in a complete growth cycle. Different responses were found in shoot height and fresh weight when the Chinese cabbages were treated with different contents of biogas slurry. The most obvious effect was observed with 25% biogas slurry. The shoot height and fresh weight significantly increased compared with those of the controls during different sampling periods. However, when the contents of biogas slurry were more than 50%, the shoot height and fresh weight were increasingly reduced with increases of biogas slurry contents. The contents of total Vc, reduced ascorbate (AsA) and dehydroascorbate (DHA) changed differently. With the 25% biogas slurry treatment, total Vc content significantly increased after 45 and 60 d treatment and AsA content also increased even though there was no significant difference compared with that in the controls. DHA content increased considerably during the different sampling periods. When the contents of biogas slurry were more 50%, the contents of total Vc, AsA and DHA were considerably reduced with increases of biogas slurry contents. In the AsA cycle, the activities of ascorbic acid peroxidase (APX), ascorbate oxidase (AAO) and dehydroascorbate reductase (DHAR) showed the same change trend, initially increasing and then decreasing with an increase of treatment duration, i.e. the activities showed the maximum values after 30 d under 25% biogas slurry, whereas the activity of monodehydroascorbate reductase (MDHAR) increased with increased treatment duration. At the same time, the contents of oxidized glutathione (GSSG) and reduced glutathione (GSH) increased significantly with an increase of treatment duration in the GSH cycle. There were significant differences compared with those in the control. The activity of glutathione reductase (GR) increased initially and then decreased with an increase of treatment duration. The activity showed a maximum value after 45 d treatment with the 25% biogas slurry. It is suggested that the optimum biogas slurry content promotes growth,, enhances AsA-GSH circulation metabolism, and maintains higher antioxidative stress capacity in Chinese cabbage.

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