Abstract: To assess cultivar differences in response to nitrogen of Fe, Mn, Cu and Zn contents and their types in triticale (Triticosecale) plants at the optimum cutting stage-heading stage, a potted plant experiment was carried out using two N levels (low and normal N) using 31 triticale cultivars from different origins. 1) Under normal nitrogen supply, Fe, Mn, Cu, Zn contents in triticale plants were significantly higher than those in plants with a low nitrogen supply. There were cultivar variations in Fe, Mn, Cu, Zn contents. Coefficients of variation were 14.16%-32.75%. In addition to the cultivar and N levels, there was a cultivar×N interaction of micronutrient contents. 2) There were also variations in the response of Fe, Mn, Cu, Zn contents in triticale plants. Based on the range and coefficients of variation of Fe, Mn, Cu, Zn contents in triticale under two N levels, the response of Fe, Mn, Cu, Zn contents in triticale plants to N levels could be classified into three types: insensitive, intermediate, and sensitive types.3) Correlation analysis showed that Fe content was not significantly correlated with shoot biomass in triticale but there were significant or highly significant correlations (coefficients of 0.36, 0.43 and 0.44, respectively) between Mn, Cu, Zn contents and shoot biomass in triticale plants. So, the cultivar×environment interaction can be manipulated to reach higher productivity and enrichment of micronutrient in triticale planting and breeding.