Isolation and identification of endogenic nitrogen-fixing bacteria in the roots of Jerusalem artichoke (Helianthus tuberosus)

Abstract

Abstract: Endogenic nitrogen-fixing bacteria (ENB) from the roots of Jerusalem artichoke (Helianthus tuberosus) were isolated and identified using acetylene reduction assay (ARA) combined with gas chromatography (GC). The seven ENB strains isolated belonged to Rhizobium (2 strains), Stenotrophomonas (3 strains), Pseudomonas (1 strain) and Enterobacter (1 strain). Significant differences in nitrogenase activity were found among the ENB strains. The nitrogenase activities of two strains, cho1 and cho9, were higher than 100 nmol/(mL·h). Cho4 strain had the strongest salt-tolerance and could grow normally in condition of 7% NaCl. Five strains showed the power of phosphate solubilization and strain cho7 had the highest ability (63.84 μg/mL). All the ENB strains could excrete IAA, but there was a significant difference in the ability to do so, and strain cho2 had the highest activity (13.49 μg/mL).

CLC Number:

Q945.13

MENG Xian-fa, LONG Xiao-hua, KANG Jian, WANG Xue-qing, LIU Zhao-pu. Isolation and identification of endogenic nitrogen-fixing bacteria in the roots of Jerusalem artichoke (Helianthus tuberosus)[J]. Acta Prataculturae Sinica, 2011, 20(6): 157-163.

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