Inhibitory effect of Trifolium pratense on the growth of Lactuca sativa seedlings

Abstract

Abstract: The inhibitory effects on Lactuca sativa of the root extracts and fractions prepared with different solvents from Trifolium pratense were tested and compared by living-bioassay methods. The ethanol extract showed a significant inhibitory effect on the germination and growth of L. sativa seeds and seedlings. The chloroform and ethylacetate extracts of the root showed stronger inhibitory effects and were determined as the effective phytotoxic fractions of T. pratense. The bioactivity-driven fractionation of the chloroform extract led to the purification of a flavonoid glycoside, which was characterized (mainly by NMR spectral elucidation) as (6aR, 11aR)-trifolirhizin. This compound led to significant reductions of fresh weight and root length of L. sativa seedlings and stimulated the activity of SOD and CAT at lower concentrations but inhibited the activity of the enzymes at higher concentrations. Reactive oxygen species increased and the peroxidation of membrane lipid was induced by (6aR, 11aR)-trifolirhizin at higher concentrations to affect the related physiological processes for the growth and development of plants.

CLC Number:

Q948.12⁺2.1

XU Rui, LIU Quan, YAN Zhi-qiang, JIN Hui, CUI Hai-yan, PU Xun, QIN Bo. Inhibitory effect of Trifolium pratense on the growth of Lactuca sativa seedlings[J]. Acta Prataculturae Sinica, 2011, 20(6): 45-51.

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