The construction of genetic linkage frame map in tetraploid Medicago using RAPD markers

Abstract

Abstract: Using random amplified polymorphic DNA (RAPD) molecular genetic markers analyze the F₂ population of 94 plant individuals. The F₂ segregating population derived from a self-pollinated F₁ hybrid individual of the cross Medicago sativa×Medicago falcata. The genetic analyses were performed by using maximum-likelihood equations and related computer programs. The genetic map comprises 74 markers, and contains 8 linkage groups covering 1 261.5 cM, with an average distance of 24.73 cM between markers. This genetic linkage map provides an entry point for the construction of saturated tetraploid alfalfa molecular genetic map and further development of alfalfa molecular genetic research.

CLC Number:

SUN Juan-juan, YAO Ting-ting. The construction of genetic linkage frame map in tetraploid Medicago using RAPD markers[J]. Acta Prataculturae Sinica, 2012, 21(1): 170-175.

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