A study on genetic diversity of Avena germplasm resources detected by ISSR

Abstract

Abstract: Genetic diversity of 42 Avena sativa varieties and 6 Avena nuda varieties from China, Canada, America, Europe and other countries was studied using inter-simple sequence repeat (ISSR) molecular markers. Eight primers detected 144 polymorphic bands, and the proportion of polymorphic fragment was 86.1%. Based on Nei’s index, the average variation coefficient of A. sativa and A. nuda were 0.203 6 and 0.201 5, and of the Shannon diversity index were 0.325 9 and 0.305 8, respectively. The similarity coefficient of all cultivars was 0.583 3-0.941 7, and genetic distance was 0.060 1-0.539 0 indicating a rich genetic diversity of Avena, with A. sativa varieties having a higher genetic diversity than that of A. nuda varieties. Based on cluster and principal component analyses of the ISSR fingerprinting data, the 48 varieties could be divided into 5 groups. In most cases, accessions from the same continent were classified into the same group. The geographical distribution of genetic diversity in A. sativa and A. nuda were divided into two groups at 0.66, which was in accordance with traditional morphologic and other molecular marker classifications. The genetic relationship revealed by ISSR was consistent with that from other sources.

CLC Number:

LIU Huan, MU Ping, ZHAO Gui-qin. A study on genetic diversity of Avena germplasm resources detected by ISSR[J]. Acta Prataculturae Sinica, 2012, 21(4): 116-124.

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