Effects of metallothionein on expression of lymphocyte apoptosis genes and the HSP70 gene of dairy cattle under heat stress

Abstract

Abstract: Effects of exogenous metallothionein on expression of lymphocyte apoptosis genes and the HSP70 gene of dairy cattle under heat stress were studied with 25 lactating Chinese Holstein cows. They were randomly divided into five groups (A, B, C, D and E, five per group), and supplemented with 0, 16.0, 24.0, 32.0 and 40.0 mg Zn-MT, respectively, by intravenous injection. Blood samples were collected at 1st, 7th and 14th day, and the levels of HSP70, Bcl-2, Bax and p53 gene expressions in lymphocytes were measured. The milk yield of the cows in group B was more than those in groups A, C, D and E by 20.94% (P＜0.05), 15.83% (P＞0.05), 10.94% (P＞0.05) and 8.85% (P＞0.05), respectively. The level of HSP70 gene expression in group D was more (P＜0.05) than that in group A, but there were no significant differences (P＞0.05) between groups A and B, C, and E. The levels of Bcl-2 gene expression of groups B, C, D, and E were more than that of the control group (A), and there was a significant difference (P＜0.05) between groups A and C. The levels of Bax gene expression in groups B and D were lower than that in group A by 22.00% (P＞0.05) and 13.00% (P＞0.05), while the levels of p53 gene expression in groups C and E were lower than that in group A by 22.00% (P＞0.05) and 34.00% (P＞0.05), respectively. Exogenous Zn-MT can improve milk producing ability and regulate the levels of HSP70, Bcl-2, Bax and p53 gene expressions in dairy cattle in a dose-dependent manner.

CLC Number:

S823.913

LUO Jia-jie, WU Zong-ming, ZHANG Bin, LI Li-li, WU Li-zhuan, CHEN Yu-guang, XIAO Ding-fu, BI Xiao-yan. Effects of metallothionein on expression of lymphocyte apoptosis genes and the HSP70 gene of dairy cattle under heat stress[J]. Acta Prataculturae Sinica, 2012, 21(4): 244-251.

References

[1] West J W. Effects of heat-stress on production in dairy cattle[J]. Journal of Dairy Science, 2003, 86:2131-2144.
[2] 李建国. 热应激对奶牛生理常值、血液生化指标、繁殖及泌乳性能的影响[J]. 河北农业大学学报, 1998, 21(4): 69-74.
[3] Gwazdauskas F C. Effects of climate on reproduction in cattle[J]. Journal of Dairy Science, 1985, 68: 1568-1578.
[4] 吴力专, 李丽立, 张彬. 金属硫蛋白在动物营养学上的应用[J]. 长江大学学报(自然科学版), 2006, 3(1): 156-160.
[5] 吴力专, 陈海燕, 张彬, 等. 外源性金属硫蛋白对中国荷斯坦奶牛产能性能和内分泌的影响[J]. 草业学报, 2011, 20(1): 183-188.
[6] Ajij H R, Kojima Y. Chemistry and biochemistry of metallothionein[J]. Experimental Biology, 1987, 52: 25-61.
[7] De S K, McMaster T, Andrews G K. Endotoxin indution of metallothionein in gene expression[J]. Journal of Biochemistry, 1990, 265(25): 1526-1538.
[8] Green E M, Adams H R. New perspectives in circultory shock: Pathophysiologic mediator of the mammalian response to endotoxemia and sepsis[J]. Journal of American Veterinary Medicine, 1992, 200(12): 1817-1826.
[9] Miura T, Muroaka S, Ogiso T, et al. Antioxidant activity of metallothionein compared with reduced glurathione[J]. Life Science, 1997, 60(21): 301.
[10] Kondoh M, Inoue Y. Specific induction of metallothionein synthesis by mitochondrial oxidative stress[J]. Life Science, 2001, 69: 2137-2146.
[11] 张彬, 谭琼, 肖兵南, 等. 外源性金属硫蛋白对奶牛生产性能和抗氧化状况的影响[J]. 草业学报, 2009, 18(6): 137-143.
[12] 张彬, 肖定福, 李丽立, 等. 金属硫蛋白在奶牛公犊体内的代谢规律研究[J]. 草业学报, 2008, 17(6): 178-184.
[13] 张彬, 薛立群, 李丽立, 等. 外源金属硫蛋白对奶牛抗热应激调控及SOD基因表达的影响[J]. 应用生态学报, 2007, 18(1): 193-198.
[14] 张彬, 谭琼, 李丽立, 等. 金属硫蛋白对奶牛血液抗氧化酶GSH-Px和CAT基因表达的影响[J]. 草业学报, 2010, 19(3): 132-138.
[15] Xu C S, Xin Z H, Yuan J Y. Advance in research on biological function and transcriptional control of heat shock proteins[J]. Developmental and Reproductive Biology, 2002, 11(2): 88-94.
[16] 宋学立, 钱令嘉, 李凤芝, 等. Bcl-2基因转染对热应激心肌细胞保护作用的机制探讨[J]. 中国应用生理学杂志, 2002, 18(4): 347-349.
[17] Santini D, Tonini G, Vecchio F M, et al. Prognostic value of Bax, Bcl-2, p53, and tunel staining in patients with radically resected ampullary carcinoma[J]. Journal of Clinical Pathology, 2005, 58(2): 159-165.
[18] Karin A, Marsha V, Mieke B B, et al. Estrous cycle dependent changes in expression and distribution of Fas, Fas ligand, Bcl-2, Bax, and active caspase-3 in the rat ovary[J]. Journal of Endocrinology, 2006, 188(2): 179-192.
[19] Sheard M A. Lonizing radiation as a response-enhancing agent for CD95-mediated apoptosis[J]. International Journal of Cancer, 2001,96(3): 213-220.
[20] 李丽立. 一种猪肝金属硫蛋白提取方法: 中国, ZL 200410013212. X[P]. 2006-04-17.
[21] 张彬, 吴力专, 李丽立. 一种猪金属硫蛋白的测定方法: 中国, ZL 200410061265. 9[P]. 2007-05-07.
[22] Lemerle C, Goddard M E. Assessment of heat stress in dairy cattle in Papua New Guinea[J]. Tropical Animal Health and Production, 1986, 18(4): 232-242.
[23] 杨毅, 梁荣嵘, 刘庆华. 轻微至中度热应激对荷斯坦奶牛生理指标及产奶性能的影响[J]. 中国农学通报, 2009, 25(24): 28-31.
[24] McGarry T J, Lindquist S. The preferential translation of Drosophila HSP70 mRNA requires sequences in the untranslated leader[J]. Cell, 2005, 42(17): 903-910.
[25] Wang S, Edens F W. Stress-induced heat shock protein synthesis in peripheral leukoeytes of turkeys, Meleagris gallopavo[J]. Comparative Biochemistry and Physiology, 1993, 106: 621-628.
[26] 邱恩惠. 慢性鼻窦炎嗅觉障碍嗅上皮中HSP70、MT及NSE的表达及意义[D]. 福州: 福建医科大学, 2009.
[27] Vaux D L, Cory S, Adams J M. Bcl-2 gene promotes haemopoietic cell survival and cooperates witn c-myc to irnrnortalize pre-B cell[J]. Nature, 1988, 335(6189): 440-442.
[28] Alvarez S, Drane P, Meiller A. A comprehensive study of p53 transcriptional activity in thymus and spleen of gamma irradiated mouse: High sensitivity of genes involved in the two main apoptotic pathways[J]. International Journal of Radiation Biology, 2006, 82(11): 761-770.
[29] Vanlandingham J W, Tassabehji N M, Somers R C, et al. Expression profiling of p53-target genes in copper-mediated neuronal apoptosis[J]. Neuromolecular Medicine, 2005,7(4): 311-324.
[30] Zhan Q, Alamo I, Yu K, et al. The apoptosis-associated gamma-ray response of Bcl-xl depends on normal p53 function[J]. Oncogene, 1996, 13(10): 2287-2293.
[31] 林如辉, 刘健. 热疗与肿瘤细胞凋亡相关基因的研究进展[J]. 实用临床医药杂志, 2006, 10(3): 12-15.
[32] 孙忠东, 高尚志, 毛志福. 金属硫蛋白对供心细胞Bcl-2、Bax和Fas蛋白表达的影响[J].中华实验外科杂志, 2005, 22(3): 375.
[33] Shimoda R, Achanzar W E, Qu W, et al. Metallothionein is a potential negative regulator of apoptosis[J]. Toxicological Sciences, 2003, 73(2): 294-300.