Abstract: This study was set up in different tobacco-growing areas in Sichuan province to study different ecological conditions on the leaf appearance quality, internal quality, and chemical composition. Leaf appearance quality of tobacco samples from Dechang, Yuanba and Yanbian were better than others, while tobacco samples from Dechang and Yanbian with good sensory quality. Determination of chemical composition of tobacco leaves showed that tobacco samples from all experimental sites were with higher leaf total sugar and reducing sugar content. In the total alkali content, all experimental sites with still relatively high content of upper leaves (basically more than 2.5%), while the lower and middle leaves with appropriate content. In total nitrogen and chlorine content of leaf samples, all were at the appropriate level within the scope of 1.5%-2.5% basically. Potassium content of all samples varied accordingly, with the highest of 2.46% and the lowest concentration of 1.50%. Leaf petroleum content of all samples were more than 2.0%, while organic acid content of most samples were greater than 16.0%.In the respect of sugar to alkali ratio, most samples were in suitable range of 8-12. The results of this study indicate that the most suitable regions for high quality tobacco production are Panzhihua and Liangshan area.