Establishment of a highly efficient regeneration system of switchgrass artificial spike buds

Abstract

Abstract: With inflorescence of three genotypes of Xiji No.1, Xiji No.2, Xiji No.3 as materials, using single factor design consider factors including genotype, panicle length, phytohormone, the length of spike buds in the initial cutting period and in mass cultivating period, the carbon source, pH, spike buds cutting way and root induction medium, to optimize the regeneration system of switchgrass (Panicum virgatum) artificial spike buds. The optimal regeneration system was: genotype Xiji No.1, panicle length 1.5 cm, 3.0 mg/L 6-BA+0.1 mg/L 2,4-D as phytohormone, the length of spike buds in the initial cutting period and in mass cultivating period was 1.5 cm, maltose as carbon source, pH 5.8, vertical spike buds cutting, and 1/2 MS as root induction medium. The proliferation efficiency of switchgrass artificial spike buds might reach up to 4 167.00%.

CLC Number:

Q945.39

CHAI Guai-qiang, XU Kai-jie, WANG Yong-feng, SUN Feng-li, SONG Li-li, LIU Shu-dong, XI Ya-jun. Establishment of a highly efficient regeneration system of switchgrass artificial spike buds[J]. Acta Prataculturae Sinica, 2012, 21(4): 98-104.

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