Effects of exogenous nitric oxide on photosynthetic and bioluminescent characteristics in ryegrass seedlings under osmotic stress

Abstract

Abstract: The regulating effect of nitric oxide (NO) on photosynthetic physiological response of herbage under osmotic stress, was studied in a hydroponic experiment. The effect of the exogenous NO donor sodium nitroprusside (SNP) on the photosynthetic pigment content, gas exchange and chlorophyll fluorescence parameters, and bioluminescence intensity in leaves of ryegrass (Lolium perenne) under osmotic stress simulated by 15% PEG-6000 were investigated. The stress from 100 μmol/L SNP significantly increased photosynthetic pigment contents, net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), stomatal limited value (L_s), PSⅡ maximal photochemical efficiency (F_v/F_m) and actual photochemical efficiency (Φ_PSⅡ), but reduced intercellular CO₂ concentration (C_i), PSⅡ excitation pressure (1-q^P), non-photochemical quenching (NPQ), ultra-weak luminescence intensity, fluorescence intensity and phosphorescence intensity in ryegrass seedling leaves. The addition of haemoglobin (Hb), a NO scavenger, reversed the effects of SNP, but application of 100 μmol/L sodium nitrate or nitrite (the decomposition products of NO or its donor SNP) or sodium ferricyanide (an analog of SNP) had no significant alleviation effects on osmotic stress. NO may alleviate the damage of osmotic stress to the leaf photosynthetic apparatus by increasing photosynthetic pigment and light-energy utilization, and decreasing bioluminescent intensity, thus improving photosynthetic efficiency of the ryegrass seedlings under osmotic stress.

CLC Number:

LIU Jian-xin, WANG Jin-cheng, WANG Rui-juan, JIA Hai-yan. Effects of exogenous nitric oxide on photosynthetic and bioluminescent characteristics in ryegrass seedlings under osmotic stress[J]. Acta Prataculturae Sinica, 2013, 22(1): 210-216.

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