Abstract: In order to explore the mechanism of Pb accumulation and transportation in plant tissue,the growth and physiological responses of turfgrass under soil Pb stress were evaluated in a pot experiment with three warm-season turfgrass species: Eremochloa ophiuroides (centipedegrass),Cynodon dactylon (bermudagrass),and Zoysia japonica (zoysiagrass). By applying Pb of different concentrations to the soil,CK(0 mg/kg)and five Pb treatments as the following 250,500,1000,2000,4000 mg/kg were set in this experiment. Our results showed that there is a significant decrease of the visual quality and leaf relative water content under 4000 mg/kg treatment comparing with control. Leaf chlorophyll a and b content significantly reduced under 4000 mg/kg treatment comparing with control among all three turfgrasses. The electrolyte leakage in leave gradually rises with the increase of Pb level among all three turfgrasses. These results suggested that cell membrane of leave is damaged by application of Pb. The antioxidant defense system (superoxide dismutase/SOD,catalase/CAT,peroxidase/POD) was greatly activated under the various Pb stress among three turfgrasses species. Under all four Pb treatments in centipedgrass,the activity of SOD gradually rises and reaches a maximum at 20 day after Pb application,and the activity of CAT at day 5 after Pb treatment is greatly higher than that in control. While the activity of POD increases significantly only under 2000 and 4000 mg/kg Pb treatments and reaches the maximum at day 15 after Pb application in centipedigrass. Under all four Pb treatments in bermudagrass,the activity of SOD rises at day 5 after Pb treatment and boosts a maximum at day 25,and the activity of CAT reaches a maximum at 15 day after treatment. While the activity of POD in Pb treatments rises gradually with the accumulation of Pb in soil and reaches a maximum at day 30 in all Pb treatments. The activity of SOD is significantly higher under 4000 mg/kg treatment than that in control and reaches a maximum at 15 day after treatment in zoysiagrass. While the activity of POD reaches a maximum at day 25 after treatment and then drops at day 30,activity of CAT shows a similar trend as that of POD and reaches a maximum at day 15 after treatment. These data implied that the protection capacity of three enzymes varied among three grass species under lead stress. Combing with the growth effect,we concluded that Pb resistance from high to low among three turfgrass species is in the following order: bermudagrass>zoysiagrass>centipedegrass.