Optimized accelerated aging test for common vetch

doi:10.11686/cyxb2017002

Abstract

Abstract: Accelerated aging is a common method for seed vigor testing, but the outcome differs with different species. In this study nine seed lots of common vetch (Vicia sativa) were assessed; seeds were aged at 100% relative humidity at 41, 43 and 45 ℃ for 12, 24, 36, 48, 60 and 72 h, respectively. An optimal accelerated aging condition was selected by analyzing germination % and rate of germination. Results indicated that aging temperature, time and their interaction showed significant differences for germination % and germination rate (P<0.01). With increasing temperature and time germination % gradually declined. Germination % decreased significantly after aging for 48 h (P<0.05) (except for seed lot 6). The germination rate increased firstly and then decreased after 24 h. Correlation analysis showed that the effects of aging time on germination % and germination rate were greater than that of temperature. Aging seed for at 41 ℃ for 60 hours was able to separate the seed lots into groups of 8 or 6 based on germination % and germination rate, respectively (P<0.05). We concluded that aging at 41 ℃ for 60 h was the optimal condition for accelerated age testing of common vetch.

MO Qing, LÜ Yan-Yan, WANG Yan-Rong. Optimized accelerated aging test for common vetch[J]. Acta Prataculturae Sinica, 2017, 26(11): 131-138.

References

[1] Hampton J G, Tekrony D M. Handbook of Vigour Test Methods[M]. 3 Ed. Zurich: The International Seed Testing Association, 1995.
[2] Li A P, Li R X, Zhang Y L. Introduction about seed vigor. Seed Science & Technology, 2009, 27(7): 17-18.
李安平, 李瑞祥, 张亚丽. 浅谈种子活力. 种子科技, 2009, 27(7): 17-18.
[3] Zhang L X, Gao Q G, Sun H Q. Discussion about how to improve seed quality supervision and testing. Seed Science & Technology, 2011, 29(4): 14-15.
张丽霞, 高群革, 孙花侨. 浅议如何搞好种子质量监督检验工作. 种子科技, 2011, 29(4): 14-15.
[4] Baalbaki R, Elias S, Marcosfilho J, et al . Seed Vigour Testing Handbook[M]. Nebraska: Association of Official Seed Analysts, 1983.
[5] Kulik M M, Yaklich R W. Evaluation of vigor tests in soybean seeds: relationship of accelerated aging, cold, sand bench, and speed of germination test to field performance. Crop Science, 1982, 22(4): 766-770.
[6] Wilson D O, Alleyne J C, Shafii B, et al . Combining vigor test results for prediction of final stand of shrunken-2 sweet corn seed. Crop Science, 1992, 32(6): 1496-1502.
[7] Khan A Z, Shah P, Mohd F, et al . Vigor tests used to rank seed lot quality and predict field emergence in wheat. Pakistan Journal of Botany, 2010, 42(5): 3147-3155.
[8] Zhu C, Zeng G W. Relationship between lipid peroxidation, endogenous polyamines and seed vigour of cucumber. Journal of Zhejiang Agricultural University, 1993, 19(2): 198-202.
朱诚, 曾广文. 黄瓜种子活力与脂质过氧化及内源多胺的关系. 浙江农业大学学报, 1993, 19(2): 198-202.
[9] Wang M X, Yi J. Effects of artificial aging on seeds vigor of Ceratoides arborescens . Chinese Journal of Grassland, 2003, 25(4): 31-35.
王明霞, 易津. 人工劣变处理对华北驼绒藜种子活力的影响. 中国草地学报, 2003, 25(4): 31-35.
[10] Zhou J, Wang Y R. Effects of artificial aging treatment on vigor index of Elymus nutans seeds. Pratacultural Science, 2011, 28(7): 1275-1279.
周晶, 王彦荣. 人工老化处理对垂穗披碱草种子活力指标的影响. 草业科学, 2011, 28(7): 1275-1279.
[11] Ghasemi E, Ghahfarokhi M G, Darvishi B, et al . The effect of hydro-priming on germination characteristics, seedling growth and antioxidant activity of accelerated aging wheat seeds. Cercetari Agronomice in Moldova, 2014, 47(4): 41-48.
[12] Ibrahim A E, Tekrony D J, Egli D B. Accelerated aging techniques for evaluating sorghum seed vigor. Journal of Seed Technology, 1993, 17(1): 29-37.
[13] Wang Y H, Wang X G, Lian J J, et al . Optimization of the accelerated ageing condition for Kentucky bluegrass seeds. Acta Agrestia Sinica, 2008, 16(6): 600-604.
王玉红, 王新国, 廉佳杰, 等. 草地早熟禾种子加速老化方法研究. 草地学报, 2008, 16(6): 600-604.
[14] Yan H F, Mao P S. Optimizing the accelerated ageing condition of Siberian wildrye seeds. Seed, 2013, 32(7): 1-6.
闫慧芳, 毛培胜. 老芒麦种子人工加速老化条件的筛选比较. 种子, 2013, 32(7): 1-6.
[15] Demir I, Ozden Y S, Yilmaz K. Accelerated ageing test of aubergine, cucumber and melon seeds in relation to time and temperature variables. Seed Science and Technology, 2004, 32(3): 851-855.
[16] Chen M J, Jia S X. Feeding Plants of China[M]. Beijing: China Agriculture Press, 2002: 673-675.
陈默君, 贾慎修. 中国饲用植物[M]. 北京: 中国农业出版社, 2002: 673-675.
[17] Chen J G. Common vetch. Rural Breeding Technology, 2003, 3: 31.
陈建纲. 箭筈豌豆. 农村养殖技术, 2003, 3: 31
[18] Wang X, Zeng Z H, Zhu B, et al . Effect of different intercropping and mixture modes on forage yield and quality of oat and common vetch. Acta Agronomica Sinica, 2007, 33(11): 1892-1895.
王旭, 曾昭海, 朱波, 等. 箭筈豌豆与燕麦不同间作混播模式对产量和品质的影响. 作物学报, 2007, 33(11): 1892-1895.
[19] Shen Y, Lin R H. A fast vigor test method for common vetch with ninhydrin paper. Plant Physiology Communications, 1981, 3: 9.
沈永, 林榕辉. 用茚三酮试纸法快速测定箭筈豌豆种子的活力. 植物生理学通讯, 1981, 3: 9.
[20] Wang Y R, Yu L, Sun J H. Forage seed testing procedure: GB/T2930.4-2001[S]. Beijing: China Standard Publisher, 2001.
王彦荣, 余玲, 孙建华. 牧草种子检验规程: GB/T2930.4-2001[S]. 北京: 中国标准出版社, 2001.
[21] Yang X, Baskin C C, Baskin J M, et al . Seed mucilage improves seedling emergence of a sand desert shrub. PLoS One, 2012, 7(4): e34597.
[22] Huang W D, Wang Y R, Hu X W. Germination responses of three desert plants to temperature and water potential. Acta Prataculturae Sinica, 2009, 18(3): 171-177.
黄文达, 王彦荣, 胡小文. 三种荒漠植物种子萌发的水热响应. 草业学报, 2009, 18(3): 171-177.
[23] Han L L, Mao P S. Optimizing the accelerated aging condition of the oat seeds. Seed, 2007, 26(11): 31-34.
韩亮亮, 毛培胜. 燕麦种子人工加速老化条件的筛选优化. 种子, 2007, 26(11): 31-34.
[24] Xie H, Chen X Z, Qi J Y, et al . Study on seed vigor of the artificial aged seeds in soybean. Journal of Beijing Agricultural College, 2006, 21(3): 15-17.
谢皓, 陈学珍, 祁佳玥, 等. 人工加速老化对大豆种子活力的影响. 北京农学院学报, 2006, 21(3): 15-17.
[25] Mao P S, Li Y F. Study on optimizing the accelerated aging condition of alfalfa seeds. Seed, 2010, 29(10): 10-14.
毛培胜, 李寅菲. 紫花苜蓿种子人工加速老化方法研究. 种子, 2010, 29(10): 10-14.
[26] Kalmbacher R S, West S H, Martin F G. Seed dormancy and aging in atra paspalum. Crop Science, 1999, 39(6): 1847-1852.
[27] Kong L Q, Wang J F, Liu G Q, et al . Physiological response of hard seeds of alfalfa on accelerated aging duration. Acta Agrestia Sinica, 2011, 19(1): 81-85.
孔令琪, 王俊峰, 刘国庆, 等. 苜蓿硬实种子对人工老化时间处理的生理响应. 草地学报, 2011, 19(1): 81-85.
[28] Siddique S B, Seshu D V, Pardee W D. Rice cultivars variability in tolerance for accelerated aging of seed. IRRI Research Paper Series-International Rice Research Institute, 1988, (131): 7.
[29] Dong G J, Hu X M, Zeng D L, et al . Compare about artificial aging and natural aging of rice. Journal of Zhejiang Agricultural Sciences, 2004, 1(1): 27-29.
董国军, 胡兴明, 曾大力, 等. 水稻种子人工老化和自然老化的比较研究. 浙江农业科学, 2004, 1(1): 27-29.
[30] Li M B, Wang H, Piao Z Z, et al . Primary study on artificial aging and storage characteristics in weedy rice. Crops, 2010, (5): 30-33.
李茂柏, 王慧, 朴钟泽, 等. 杂草稻人工老化和耐储藏特性的初步研究. 作物杂志, 2010, (5): 30-33.
[31] Liu M J, Wang T G, Chen S L, et al . Physiological and seed vigour changes of maize seeds during artificial aging course. Journal of Nuclear Agricultural Sciences, 2008, 22(4): 510-513.
刘明久, 王铁固, 陈士林, 等. 玉米种子人工老化过程中生理特性与种子活力的变化. 核农学报, 2008, 22(4): 510-513.
[32] Kong L Q, Huo H Q, Mao P S. Antioxidant response and related gene expression in aged oat seed. Frontiers in Plant Science, 2015, 6: 158.