Isolation and identification of an inorganic phosphorus-solubilizing bacterium RW8 and its growth-promoting effect on white clover (Trifolium repens)

doi:10.11686/cyxb2016282

Abstract

Abstract: Nine strains of inorganic phosphorus-solubilizing bacteria were isolated from the rhizosphere of white clover (Trifolium repens), and each strain was identified to the genus and species level based on its phenotypic and cytological characteristics. The growth-promoting effect of each strain on white clover was also analyzed. Strain RW8, which showed the strongest phosphorus-solubilizing activity, was selected based on analyses of the size and ratio of its plaques and colonies. Scanning electron microscopy observations revealed that RW8 was Bacillus brevis, a flagellated bacterium. Colonies of RW8 were opaque and had a smooth surface. In NCBI blast searches, the 16S rDNA sequence of RW8 showed 99.5% homology with that of Enterobacter sp. A Biolog analysis suggested that RW8 had high similarity (0.610) with Enterobacter cloacae. In a phosphorous-dissolving assay, RW8 produced 424.85 μg/mL dissolved phosphorus, which was possibly related to its strong organic acid production (12.30 μg/mL). RW8 strongly affected the biomass distribution to roots in white clover seedlings. Root elongation and the fresh weight and dry weight of roots were lower in RW8-colonized plants than in control plants, although the differences were not statistically significant. However, the seedling biomass (dry weight and fresh weight) of RW8-colonized white clover was significantly greater than that of control plants (P<0.05), and this phenomenon was not a result of indole acetic acid production by the bacterium. Further research is required to determine the precise mechanism of its growth-promoting effect.

CLC Number:

lixiaodongzl@163.com

CAI Lu, WANG Xiao-Li, CHEN Yin, WANG Zi-Yuan, LI Xiao-Dong. Isolation and identification of an inorganic phosphorus-solubilizing bacterium RW8 and its growth-promoting effect on white clover (Trifolium repens)[J]. Acta Prataculturae Sinica, 2017, 26(5): 181-188.

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