Characteristics of organic acids accumulation and oxalate metabolism in Kochia sieversiana under salt and alkali stresses

doi:10.11686/cyxb2016365

Abstract

Abstract: In this study, seedlings of Kochia sieversiana were subjected to different salt and alkali stresses. The characteristics of organic acid accumulation and mechanisms regulating oxalate (OXA) metabolism were investigated by quantifying various organic acids and determining the activities of OXA metabolism-related enzymes. Seven kinds of organic acids (including OXA) accumulated in K. sieversiana under extended alkali stress. The accumulation of these organic acids was correlated with alkali stress (high pH), suggesting that they play roles in ion balance and pH regulation. The degradation pathway of L-ascorbic acid was not the main source of OXA. The cleavage of oxaloacetate by phosphoenolpyruvate carboxylase (PEPcase) probably played a minor role in OXA synthesis, but glycolate oxidase (GO) was the key enzyme for OXA synthesis. The activity of oxalate oxidase (OxO) involved in OXA decomposition was not a limiting factor for endogenous OXA accumulation. Taken together, these results showed that OXA accumulation in K. sieversiana under alkali stress was not because of reduced OXA degradation by OxO, but largely depended on OXA synthesis by GO.

MA Ying, WANG Xiao-Ping, JIANG Hai-Bo, SHI De-Cheng. Characteristics of organic acids accumulation and oxalate metabolism in Kochia sieversiana under salt and alkali stresses[J]. Acta Prataculturae Sinica, 2017, 26(7): 158-165.

References

[1] Franceschi V R, Nakata P A. Calcium oxalate in plants: formation and function. Annual Review of Plant Biology, 2005, 56: 41-71.
[2] Volk G M, Goss L J, Franceschi V R. Calcium channels are involved in calcium oxalate crystal formation in specialized cells of Pistia stratiotes L. Annals of Botany, 2004, 93: 741-753.
[3] Ström L, Owen A G, Godbold D L, et al . Organic acid mediated P mobilization in the rhizosphere and uptake by maize roots. Soil Biology and Biochemistry, 2002, 34: 703-710.
[4] López-Bucio J, Nieto-Jacobo M F, Ramrez-Rodrguez V, et al . Organic acid metabolism in plants: From adaptive physiology to transgenic varieties for cultivation in extreme soils. Plant Science, 2000, 160: 1-13.
[5] Ma Y, Guo L Q, Zhang S F, et al . Solutes accumulation and distribution traits of an alkali resistant forage plant Kochia sieversiana and physiological contribution of organic acid under salt and alkali stresses. Acta Prataculturae Sinica, 2013, 22(1):193-200.
麻莹, 郭立泉, 张淑芳, 等. 盐碱胁迫下抗碱牧草碱地肤溶质积累、分布特点及有机酸的生理贡献. 草业学报, 2013, 22(1): 193-200.
[6] Yang J L, Zheng S J, He Y F, et al . Comparative studies on the effect of a protein-synthesis inhibitor on aluminium-induced secretion of organic acids from Fagopyrum esculentum Moench and Cassia tora L. roots. Plant Cell Environment, 2006, 29: 240-246.
[7] Yang H, Yang Z M, Zhou L X, et al . Ability of Agrogyron elongatum to accumulate the single metal cadmium, copper, nickel and lead and root exudation of organic acids. Journal of Environmental Sciences, 2001, 13: 368-375.
[8] Ma Y, Guo L Q, Wang H X, et al . Accumulation, distribution, and physiological contribution of oxalic acid and other solutes in an alkali-resistant forage plant, Kochia sieversiana , during adaptation to saline and alkaline conditions. Journal of Plant Nutrition and Soil Science, 2011, 174(4): 655-663.
[9] Zhai Y J, Feng X H, Kang Y G, et al . Pharmacognostical identification of fructus Kochia sieversiana . Journal Chinese Materia Medica, 1996, 19(6): 283-285.
翟延君, 冯夏红, 康延国, 等. 碱地肤的生药鉴定. 中药材, 1996, 19(6): 283-285.
[10] Peng B, Xu W, Shao R, et al . Growth of Suaeda salsa tin response to salt stress in different habitats. Acta Prataculturae Sinica, 2016, 25(4): 81-90.
彭斌, 许伟, 邵荣, 等. 不同生境种源盐地碱蓬幼苗生长发育对盐分胁迫的响应和适应. 草业学报, 2016, 25(4): 81-90.
[11] Xiao G Z, Teng K, Li L J, et al . Antioxidant enzyme activity and gene expression in creeping bentgrass under salt stress. Acta Prataculturae Sinica, 2016, 25(9): 74-82.
肖国增, 滕珂, 李林洁, 等. 盐胁迫下匍匐翦股颖抗氧化酶活性及基因表达机制研究. 草业学报, 2016, 25(9): 74-82.
[12] Shi D C, Yin L J. Difference between salt (NaCl) and alkaline (Na 2 CO 3 ) stresses on Puccinellia tenuiflora (griseb.) scribn. et merr. plants. Acta Botanica Sinica, 1993, 35(2): 144-149.
石德成, 殷立娟. 盐(NaCl)与碱(Na 2 CO 3 )对星星草胁迫作用的差异. 植物学报, 1993, 35(2): 144-149.
[13] GB/T5009.86-2003, Determination of Total Ascorbic Acid in Fruits, Vegetables and Derived Products (Fluorometric method and Colorimetric Method)[S]. Beijing: China Standards Press, 2004.
GB/T5009.86-2003, 蔬菜、水果及其制品中总抗坏血酸的测定(荧光法和2, 4-二硝基苯肼法)[S]. 北京: 中国标准出版社, 2004.
[14] Booker F L, Reid C D, Brunschon Harti S, et al . Photosynthesis and photorespiration in soybean [ Glycine max (L.) Merr.] chronically exposed to elevated carbon dioxide and ozone. Journal of Experimental Botany, 1997, 48: 1843-1852.
[15] Yang Z M, Yang H, Wang J, et al . Aluminum regulation of citrate metabolism for Al-induced citrate efflux in the roots of Cassia tora L. Plant Science, 2004, 166: 1589-1594.
[16] Brock M, Darley D, Textor S, et al . 2-methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans : characterization and comparison of both enzymes. European Journal of Biochemistry, 2001, 268: 3577-3586.
[17] Thakur M, Goyal L, Pundir C S. Discrete analysis of plasma oxalate with alkylamine glass bound sorghum oxalate oxidase and horseradish peroxidase. Journal of Biochemical and Biophysical Methods, 2000, 44: 77-88.
[18] Bradford M M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.
[19] Shi D C, Yin S J, Yang G H, et al . Citric acid accumulation in an alkali-tolerant plant Puccinellia tenuiflora under alkaline stress. Acta Botanica Sinica, 2002, 44: 537-540.
[20] Alhendawi R A, Römheld V, Kirby E A, et al . Influence of increasing bicarbonate concentration on plant growth, organic acid accumulation in roots and iron uptake by barley, sorghum and maize. Plant Nutrition, 1997, 20: 1731-1753.
[21] Shao H B, Liu Z, Zhang Z B, et al . Biological roles of crop NADP-malic enzymes and molecular mechanisms involved in abioticstress. African Journal of Biotechnology, 2011, 10(25): 4947-4953.
[22] Guo L Q, Shi D C, Wang D L. The key physiological response to alkali stress by the alkali-resistant halophyte Puccinellia tenuiflora is the accumulation of large quantities of organic acids and into the rhyzosphere. Journal of Agronomy and Crop Science, 2009, 196: 123-135.
[23] Lv J Q, Li C Y, Yang C W, et al . Effect of natural saline soil on organic acid accumulation in the stem and leaf of Chloris virgata and analysis of stress factors. Acta Prataculturae Sinica, 2015, 24(4): 95-103.
吕家强, 李长有, 杨春武, 等. 天然盐碱土壤对虎尾草茎叶有机酸积累影响及胁迫因子分析. 草业学报, 2015, 24(4): 95-103.
[24] Libert B, Franceschi V R. Oxalate in crop plants. Journal of Agricultural and Food Chemistry, 1987, 35: 926-938.
[25] Millerd A, Morton R, Wells J R E. Role of isocitrate lyase in synthesis of oxalic acid in plants. Nature, 1962, 196: 955-956.
[26] Chang C C, Beevers H. Biogenesis of oxalate in plant tissues. Plant Physiology, 1968, 43: 1821-1828.
[27] Hurkman W J, Tanaka C K. Effect of salt stress on germin gene expression in barley roots. Plant Physiology, 1996, 110: 971-977.