Identification and biological attributes of seed-borne bacteria isolated from lucerne cv. Gannong No.3

doi:10.11686/cyxb2017107

Abstract

Abstract: Eight bacterial strains (ZSR9-16) were isolated from surface-sterilized seeds of Gannong No.3 lucerne using the dilution plate method. Biological functions including indole acetic acid (IAA) production, nitrogen fixation, phosphate solubilization and ability to decompose cellulose were determined for all strains. The results showed that six strains (ZSR9-12 and ZSR14-15) had the ability to produce IAA, solubilize phosphorus, fix nitrogen and decompose cellulose; ZSR9 was better than other strains. Strain ZSR13 had weak IAA production. ZSR16 had good IAA production, phosphorus solubilization, cellulose decomposition and nitrogen fixation. The morphology, phenotype and 16S rDNA sequence of all 8 strains were analyzed and revealed that they belonged to three different genera. Six Bacillus strains included B. subtilis ZSR9, B. safensis ZSR10, B. mojavensis ZSR11, B. licheniformis ZSR12, B. pumilus ZSR14 and B. atrophaeus ZSR15. ZSR13 and ZSR16 belonged to Enterobacter sp. and Terribacillus sp., respectively. These strains will be potentially very useful for improving production of lucerne.

ZHANG Zhen-fen, SHI Shang-li. Identification and biological attributes of seed-borne bacteria isolated from lucerne cv. Gannong No.3[J]. Acta Prataculturae Sinica, 2018, 27(1): 152-160.

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