Development of a plant regeneration system via tissue culture in Puccinellia tenuiflora

doi:10.11686/cyxb20140643

Abstract

Abstract: The effects of husk-off treatment and various hormone ratios on callus induction and differentiation were investigated using mature seeds of Puccinellia tenuiflora as explants. Finally, a plant regeneration system via tissue culture was established. The husk-off treatment increased seed germination rate and deceased contamination rate significantly. The optimal medium for callus induction was MS medium supplemented with 5.0 mg/L 2,4-D with the highest callus induction rate of 55.2%. The optimal medium for bud differentiation and rooting was MS medium supplemented with 1.0 mg/L 6-BA+0.5 mg/L NAA with the highest bud differentiation rate of 31.7%, the highest rooting rate of 81.7% within a period of two weeks. The rooting rate of regenerated plants was up to 100% on half strength MS medium and their transplant survival rate in soil was also up to 100%. The whole process of plant regeneration via tissue culture lasted for about 16 weeks totally. The establishment of plant regeneration system via tissue culture in P. tenuiflora has laid a foundation for further exploring the molecular mechanism of its salt tolerance.

CLC Number:

S812

WANG Xue-fang,WANG Chun-mei,ZHANG Jin-lin,DUAN Li-jie,WANG Suo-min. Development of a plant regeneration system via tissue culture in Puccinellia tenuiflora[J]. Acta Prataculturae Sinica, 2014, 23(6): 355-360.

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