Changes of soil microbial biomass and enzymatic activities among restoration stages of Langshan Forest Park, Hunan Province

doi:10.11686/cyxb20140117

Abstract

Abstract: The study on soil microbial biomass and the enzyme activities including urease,acidic phosphatase,catalase,protease,amylase and sucrase was carried out in shrub,shrub-tree and tree of plant communities in Langshan Forest Park in Hunan,China. In each forest,the tested soil was fetched back from different depths,0-20 cm and 20-40 cm. The results indicated that the content of organic matter (OM),total nitrogen (TN) and total phosphorus (TP) were decreased with soil depth increasing. The content of soil microbial biomass carbon (SMBC),microbial biomass nitrogen (SMBN),microbial biomass phosphorus (SMBP),and the contribution rate between SMBC/OM,SMBN/TN,and SMBP/TP in the 0-20 cm soil were higher than that in 20-40 cm. Among the stages of A,B and C,the content of SMBC and SMBP in stage of tree were the highest. The trend of the content of SMBP in 0-20 cm and 20-40 cm was C>A>B. When we came to the enzyme activities in the stage of A,B and C of the Langshan Forest Park plant communities,the activity of urease,acidic phosphatase,catalase,protease,amylase and sucrase in soil layer 0-20 were higher than that in soil layers 20-40 cm. The activity of sucrase in soil layer 0-20 cm was 15.14%-35.69%,higher than that in soil layer 20-40 cm. The trend of the activity of urease in soil layer 0-20 cm was C>B>A and it shown significant difference in this layer. The activity of protease in the stage of bush was 1.244 and 1.442 fold and 1.680 and 1.713 fold in that of stage of shrub-tree and tree respectively. The activity of neutral phosphatase is highest in the stage of shrub-tree while the activity of alkaline phosphatase in the stage of shrub was highest. The activity of catalase shown no significant difference among different recovery stage of A,B and C in soil layer 0-20 cm.

CLC Number:

S154.3

YANG Xian-jun,WANG Ye-she,DUAN Lin-dong,CHEN Li-jun,YAN Xue-qing. Changes of soil microbial biomass and enzymatic activities among restoration stages of Langshan Forest Park, Hunan Province[J]. Acta Prataculturae Sinica, 2014, 23(1): 142-148.

References

Reference:
[1]Liu M Q, Hu F, He Y Q, et al. Seasonal dynamics of soil microbial biomass and its significance to indicate soil quality under different vegetations restored on degaded red soils[J]. Acta Pedologica Sinica, 2003, 40(6): 937-944.
[2]Wei Y W, Su Y R, Chen X B, et al. Effects of human disturbance on profile distribution of soil organic C,total N,total P and microbial biomass in Karst Region of Northwest Guangxi[J]. Journal of Soil and Water Conservation, 2010, 24(3): 164-169.
[3]Yi H Y, Gong Y B, Wu W H, et al. Study on soil microorganism and soil enzyme activity of different vegetation in the mountain Forests-the Arid Valley ecotone in the upper reach of Minjiang River[J]. Journal of Soil and Water Conservation, 2010, 24(3): 145-149.
[4]Yang C D, Long R J, Chen X R, et al. Seasonal dynamics in soil microbial biomass and enzymatic activities under different alpine brushlands of the Eastern Qilian Mountains[J]. Acta Prataculturae Sinica, 2011, 20(6): 135-142.
[5]Yu F M, Liu H, Liu K H, et al. Changes of soil enzyme activities in the early period restoration of subalpine coniferous forests in western Sichuan, China[J]. Ecology and Environment Sciences, 2012, 21(1): 64-68.
[6]Li W, Yu L J, Li T, et al. Seasonal and spatial dynamics of soil enzyme activities and its relationship to soil fertility in Karst ecosystem——a case study of Guilin Yaji Karst experimental site[J]. Journal of Agro-Environment Science, 2008, 27(1): 260-266.
[7]Fu Y H, Huang Z S, Yu L F. Soil enzyme activities in the type of root underground habitat typical of Karst areas[J]. Acta Pedologica Sinica, 2012, 49(6): 1202-1209.
[8]Chen Y D, Jiang Y P, Zhu Y H. Differences of natural characteristics between two typical karst ecosystems in Lijiang River Basin[J]. Journal of Natural Resources, 2003,18(2): 326-332.
[9]Li X K, He C X, Tang J S, et al. Evolution and ecological processes of Karst ecosystem of Guangxi[J]. Guangxi Sciences, 2008, 15(1): 80-86, 91.
[10]Chen S Y, Liu W J, Ye B S, et al. Species diversity of vegetation in relation to biomass and environmental factors in the upper area of the Shule River[J]. Acta Prataculturae Sinica, 2011, 20(3): 70-83.
[11]Ma J M, Wu M, Zhan T T, et al. Changes of species composition and diversity among restoration stages of Loropetalum chinense communities in karst area of Lijiang River valley[J]. Ecology and Environment Sciences, 2013, 22(1): 66-71.
[12]Yang Y, Luo Y. Carbon:nitrogen stoichiometry in forest ecosystems during stand development[J]. Global Ecology and Biogeography, 2011, 20: 354-361.
[13]Bao S D. Agrochemical soil analysis[M]. Beijing: China Agriculture Press, 1999： 103-105.
[14]Wang H, Wang G, Huang Y Y, et al. The effects of pH change on the activities of enzymes in an acid soil[J]. Ecology and Environment, 2008, 17(6): 2401-2406.
[15]Luo M, Wen Q K, Ji C Y, et al. Influence of different fertilization measures on microbial biomass and activities in cotton soil[J]. Soils, 2002, 1: 53-55.
[16]Gu L J, Xu B L, Liang Q L, et al. Impact and colonisation ability of Trichoderma biocontrol on lawn soil microflora[J]. Acta Prataculturae Sinica, 2013, 22(3): 321-326.
[17]Chen X Y, Lv J L, Zhang H, et al. Studies on soil micro biomass and organic acid under different vegetations[J]. Agricultural Research In The Arid Areas, 2008, 26(3): 167-170．
[18]You M Y, Han X Z, Liang Y. The dynamics of microbial biomass carbon under different vegetations cover[J]. Chinese Journal of Soil Science, 2012, 43(6): 1401-1404.
[19]Xie S, Liu G B, Dai Q H, et al. Evolution of soil microbial biomass in the restoration process of artificial Robinia pseudoacacia under erosion environment[J]. Acta Ecologica Sinica, 2007, 27(3): 909-917．
[20]An S S, Huang Y M, Liu M Y, et al. The responses and evaluation of soil enzymatic activities to plant rehabilitation in Ningxia Loess Hilly-gully region[J]. Research of Soil and Water Conservation, 2005, 12(3): 31-34．
[21]Wang W, Jiang W L, Xie Z K, et al. Study on soil water in rhizosphere and root system distribution of Nitraria tangutorum on Loess Plateau[J]. Acta Prataculturae Sinica, 2013, 22(1): 20-28.
[22]Teng Z Q, Li X D, Han H G, et al. Effects of land use patterns on soil phosphorus fractions in the Longzhong part of the Loess Plateau[J]. Acta Prataculturae Sinica, 2013, 22(2): 30-37.

参考文献:
[1]刘满强, 胡锋, 何园球, 等. 退化红壤不同植被恢复下土壤微生物量季节动态及其指示意义[J]. 土壤学报, 2003, 40(6): 937-944.
[2]魏亚伟, 苏以荣, 陈香碧, 等. 人为干扰对桂西北喀斯特生态系统土壤有机碳、氮、磷和微生物量剖面分布的影响[J]. 水土保持学报, 2010, 24(3): 164-169.
[3]易海燕, 宫渊波, 伍维翰, 等. 岷江上游山地森林/干旱河谷交错带植被恢复对土壤微生物量及酶活性的影响[J]. 水土保持学报, 2010, 24(3): 145-149.
[4]杨成德, 龙瑞军, 陈秀蓉, 等. 东祁连山高寒灌丛草地土壤微生物量及土壤酶季节性动态特征[J]. 草业学报, 2011, 20(6): 135-142.
[5]于方明, 刘华, 刘可慧, 等. 川西亚高山暗针叶林恢复初期土壤酶活性研究[J]. 生态环境学报, 2012, 21(1): 64-68.
[6]李为, 余龙江, 李涛, 等. 岩溶生态系统土壤酶活性的时空动态及其与土壤肥力的关系[J]. 农业环境科学学报, 2008, 27(1): 260-266.
[7]符裕红, 黄宗胜, 喻理飞. 岩溶区典型根系地下生境类型中土壤酶活性研究[J]. 土壤学报, 2012, 49(6): 1202-1209.
[8]陈余道, 蒋亚萍, 朱银红. 漓江流域典型岩溶生态系统的自然特征差异[J]. 自然资源学报, 2003,18(2): 326-332.
[9]李先琨, 何成新, 唐建生, 等. 广西岩溶山地生态系统特征与恢复重建[J]. 广西科学, 2008, 15(1): 80-86, 91.
[10]陈生云, 刘文杰, 叶柏生, 等.疏勒河上游地区植被物种多样性和生物量及其与环境因子的关系[J]. 草业学报, 2011, 20(3): 70-83.
[11]马姜明, 吴蒙, 占婷婷, 等. 漓江流域岩溶区檵木群落不同恢复阶段物种组成及多样性变化[J]. 生态环境学报, 2013, 22(1): 66-71.
[12]Yang Y, Luo Y. Carbon:nitrogen stoichiometry in forest ecosystems during stand development[J]. Global Ecology and Biogeography, 2011, 20: 354-361.
[13]鲍士旦. 土壤农化分析手册[M]. 北京: 中国农业出版社(第三版), 1999： 103-105.
[14]汪涵, 王果, 黄颖颖, 等. pH变化对酸性土壤酶活性的影响[J]. 生态环境, 2008, 17(6): 2401-2406.
[15]罗明, 文启凯, 纪春燕, 等. 不同施肥措施对棉田土壤微生物量及其活性的影响[J]. 土壤, 2002, 1: 53-55.
[16]古丽君, 徐秉良, 梁巧兰, 等. 生防木霉对草坪土壤微生物区系的影响及定殖能力研究[J]. 草业学报, 2013, 22(3): 321-326.
[17]陈小燕, 吕家珑, 张红, 等. 子午岭不同植被类型土壤微生物量与有机酸含量[J]. 干旱地区农业研究, 2008, 26(3): 167-170．
[18]尤孟阳, 韩晓增, 梁尧. 不同植被覆盖下土壤微生物量碳动态变化[J]. 土壤通报, 2012, 43(6): 1401-1404.
[19]薛萐, 刘国彬, 戴全厚, 等. 侵蚀环境生态恢复过程中人工刺槐林(Robinia pseudoacacia)土壤微生物量演变特征[J]. 生态学报, 2007, 27(3): 909-917．
[20]安韶山, 黄懿梅, 刘梦云, 等.宁南宽谷丘陵区植被恢复中土壤酶活性的响应及其评价[J]. 水土保持研究, 2005, 12(3): 31-34．
[21]王文, 蒋文兰, 谢忠奎, 等. 黄土丘陵地区唐古特白刺根际土壤水分与根系分布研究[J]. 草业学报, 2013, 22(1): 20-28.
[22]滕泽琴, 李旭东, 韩会阁, 等.土地利用方式对陇中黄土高原土壤磷组分的影响[J]. 草业学报, 2013, 22(2): 30-37.