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Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (1): 266-275.DOI: 10.11686/cyxb20140132

• Orignal Article • Previous Articles     Next Articles

Characterization and identification of Weissella species isolated from Kobresia littledalei growing in alpine meadows

YANG Yang1,SHI Chao2,GUO Xu-sheng2,3   

  1. 1.College of Pastoral Agriculture Science and Technology,State Key Laboratory of Grassland Agro-ecosystems,Lanzhou University,Lanzhou 730020,China;
    2.School of Life Science,Lanzhou University,State Key Laboratory of Grassland Agro-ecosystems,Lanzhou 730000,China;
    3.International Centre for Tibetan Plateau Ecosystem Management,Lanzhou University,Lanzhou 730000,China
  • Received:2012-02-17 Online:2014-02-20 Published:2014-02-20

Abstract: To isolate and screen optimal lactic acid bacteria (LAB) in alpine zones,conventional identification methods and 16S rRNA sequence analysis were used to identify isolated lactic acid bacteria attached to Kobresia littledalei in the Qinghai-Tibetan Plateau. A total number of 17 lactic acid bacterial strains were isolated in this study,of which 6 strains were identified as Weissella confusa and 11 strains as Weissella cibaria. The isolated strains grew well at 4℃. From 17 strains,five were able to grow at pH 3. With the exception of one strain,the other W. confusa strains could utilize arabinose for fermentation,and all W. cibaria could ferment galactose. The characteristics of Weissella in alpine zones included low temperature resistance,high acid resistance and utilization of a wide variety of carbon sources during fermentation.

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