Research progress and prospects for controlling plant diseases using Pseudomonas fluorescens

doi:10.11686/cyxb20150421

Abstract

Abstract: Biological control agents, especially Pseudomonas fluorescens, have become one of the most important and useful options for plant disease management. This study reviews the culture characteristics of P. fluorescens, including the morphological characteristics, physiological and biochemical characteristics, culture conditions and the similarities or differences with other bacteria also isolated from various plant groups to control diseases. The disease control efficiency of P. fluorescens when used alone or together with other bacteria or treatment preparations is summarized. The modes of action of both P. fluorescens and bio-active substances used for controlling plant diseases are elucidated. Hazards of P. fluorescens in medicine, agriculture and environmental protection are discussed. The outlook and prospects for biological control in the field are also evaluated and a field risk assessment presented.

SUN Guang-Zheng, YAO Tuo, ZHAO Gui-Qin, LU Hu, MA Wen-Bin. Research progress and prospects for controlling plant diseases using Pseudomonas fluorescens[J]. Acta Prataculturae Sinica, 2015, 24(4): 174-190.

References

Huang Y, Zhang Z Y, Huang L L, et al. Opinions on definition, analysis of advantages and disadvantages and integrated management of plant diseases. Plant Protection, 2009, 35(1): 97-101.
Yan W R, Zhao T C, Xiao T B, et al. Applications of biocontrol bacterial in plant disease control. Genomics and Applied Biology, 2013, 32(4): 533-539.
Liu Q, Liu Y, He Y Q, et al. Review of biological control of plant diseases. Anhui Agricultural Science Bulletin, 2012, 18(7): 67-69.
Kerr A. Biological control of crown gall through production of agrocin 84. Plant Disease, 1980, 64: 25-30.
He L Y. Advances of research and application of bacteria in biological control of plant diseases. Chinese Journal of Biological Control, 1985, 1(3): 28-31.
Ma C T, Hu Q, Yang D K. Progress of the prevention and treatment of the profitable soil microorganism for plants diseases. Shangdong Science, 2007, 20(6): 61-67.
Buchanan R E, Bergey D H. Bergey’s Manual of Determinative Bacteriology, 8th Chinese edn. Beijing: Chinese Science Press, 1984.
Dong X Z, Cai M Y. Common Bacterial System Identification Manual. Beijing: Chinese Science Press, 2002, 162-166.
Tan Z J, Xiao L,Xie B Y, et al. Microecological functions of Pseudomonad. Acta Agriculturae Nucleatae Sinica, 2004, 18(1): 72-76.
Yang Y, Li Z, Gao L X, et al. The antibiotic metabilites genes of Pseudomonas fluorescens. China Biotechnology, 2012, 32(8): 100-106.
Almario J, Prigent-Combaret C, Muller D, et al. Effect of clay mineralogy on iron bioavailability and rhizosphere transcription of 2, 4-diacetylphloroglucinol biosynthetic genes in biocontrol Pseudomonas protegens. Molecular Plant-Microbe Interactions, 2013, 26(5): 566-574.
Wang P, Li H, Qiu Y X, et al. Siderophores produced by Pseudomonas fluorescens P13 against Sclerotinia sclerotiorum. Journal of Shanghai Normal University (Natural Sciences), 2010, 39(2): 200-203.
Barahona E, Navazo A, Martínez-Granero F, et al. Pseudomonas fluorescens F113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens. Applied and Environmental Microbiology, 2011, 77(15): 5412-5419.
Nagarajkumar M, Jayaraj J, Muthukrishnan S, et al. Detoxification of oxalic acid by Pseudomonas fluorescens strain PfMDU2: implications for the biological control of rice sheath blight caused by Rhizoctonia solani. Microbiological Research, 2005, 160(3): 291-298.
Diyansah B, Aini L Q, Hadiastono T. The effect of PGPR (Plant Growth Promoting Rhizobacteria) Pseudomonas fluorescens and Bacillus subtilis on leaf mustard plant (Brassica juncea L.) infected by TuMV (turnip mosaic virus). Journal of Tropical Plant Protection, 2014, 1(1): 30-38.
Yuan L L. Optimization for Fermentation Conditions of Phenazing-1-carboxylic Acid in Genetically Engineered Bacteria M18Q. Shanghai: Shanghai Jiao Tong University, 2008.
Zhang W Q, Nie M, Xiao M. Advances in biocontrol mechanism of Pseudomonas fluorescens. Journal of Biology, 2007, 24(3): 9-11, 24.
Yang H J, Tan Z J, Xiao Q M, et al. Biocontrol functions of Pseudomonad. Chinese Journal of Eco-Agriculture, 2004, 12(3): 158-161.
Haas D, Défago G. Biological control of soil-borne pathogens by fluorescent pseudomonads. Microbiology, 2005, 3(4): 7-319.
Lv Z B, Li M Q, Hui N N, et al. Pathogen identification of water spot disease on Chinese angelica in Dingxi city of Gansu Province and pathogenicity test. Plant Protection, 2013, 39(2): 45-49.
Li H B, Peng J, Bao Y, et al. Preliminary studies on isolation and screening of Fluorescent Pseudomonas spp. for biocontrol Sclerotinia Sclerotiorum. Chinese Agricultural Science Bulletin, 2005, 21(11): 334-337.
Zhang P, Li L, Wang Z X, et al. Screening & identification of fine anti-tomato-gray-mold (Botrytis cinerea) strain. Journal of Microbiology, 2012, 32(6): 42-46.
Dong G J, Li W Y, Liu C P, et al. Identification and characterization of antagonistic bacterial strain P-72-10 against Phytophthora nicotianae. Acta Phytopathologica Sinica, 2012, 42(3): 297-305.
Chen S Y, Chen Z Y, Zhang R S. Screening and evaluation of antagonistic bacteria against Rhizoctonia solani. Acta Phytophylacica Sinica, 2013, 40(3): 211-218.
Wang X J, Bu C Y, Jin Y S, et al. Identification and inhibitory effects of antagonistic bacteria against strawberry root rot (Fusarium oxysporum). Acta Horticulturae Sinica, 2011, 38(9): 1657-1666.
Li H L. Screening of Pseudomonas fluorescens and Biocontrol of Alternaria alternata. Heilongjiang: Heilongjiang Bayi Agricultural University, 2008.
Li A R, An D R. Experiments on the screening of two antagonistic strains of Pseudomonas fluorescent in the laboratory. Journal of Microbiology, 2003, 23(4): 11-13.
Lau J, Tran C, Licari P, et al. Development of a high cell density fedback bioprocess for the heterologous production of 6-deoxyery thronolide B in E. coli. Journal of Biotechnology, 2004, 110:95-103.
Tang C M, Chen X R, Yao T, et al. Determination of the optimum cultural condition for nine PGPR strains. Grassland and Turf, 2005, (3): 27-30.
Farhangi M B, Safari Sinegani A A, Mosaddeghi M R, et al. Survival of Pseudomonas fluorescens CHA0 in soil; impact of calcium carbonate and temperature. Arid Land Research and Management, 2014, 28(1): 36-48.
Zan J Q, Lin W T. Screening and identification of a kind of flocculants-producing bacteria and its cultural conditions. Microbiology, 2010, 37(4): 547-552.
Zhao S L, Ren F E, Liu J L, et al. Screening, identification and optimization of fermentation conditions of an antagonistic actinomycetes strain to Setosphaeria turcica. Acta Microbiologica Sinica, 2012, 52(10): 1228-1236.
Yanes M L, De La Fuente L, Altier N, et al. Characterization of native fluorescent Pseudomonas isolates associated with alfalfa roots in Uruguayan agroecosystems. Biological Control, 2012, 63(3): 287-295.
Lagzian A, Saberi Riseh R, Khodaygan P, et al. Introduced Pseudomonas fluorescens VUPf5 as an important biocontrol agent for controlling Gaeumannomyces graminis var. tritici the causal agent of take-all disease in wheat. Archives of Phytopathology and Plant Protection, 2013, 46(17): 2104-2116.
Liu N, Wang K. Isolation and identification of antagonistic ginger endophytic bacteria for Aspergillus niger. Chinese Agricultural Science Bulletin, 2013, 29(4): 57-61.
Wan X Q, Guo Z K, Yan P Q, et al. Screening of antagonistic strains to tobacco anthracnose. Microbiology, 2008, 35(11): 1727-1731.
Rong L Y, Yao T, Zhao G Q, et al. Screening of siderophore-producing PGPR bacteria and their antagonism against the pathogens. Plant Protection, 2011, 37(1): 59-64.
Shen L, Wang F, Yang J, et al. Control of tobacco mosaic virus by Pseudomonas fluorescens CZ powder in greenhouses and the field. Crop Protection, 2014, 56: 87-90.
Qiao C, Chen X, Zhang Y J, et al. Laboratory and field control effects of Pseudomonas fluorescens PF7-5 against Pseudomonas syringae pv. tabaci. Forest by Product and Speciality in China, 2010, 108(5): 18-20.
Li H L, Wan X Q, Yan P Q, et al. Antagonistic action of Pseudomonas fluorescens strain G20-9 to Alternaria alternate keissler. Tobacco Science & Technology, 2008, 249(4): 56-59.
Gu J G, Fang D H, Li T F, et al. Mechanisms of Pseudomonas fluorescens RB-42 and RB-89 in biocontrol of Phytophthora parasitica var. nicotianae. Chinese Journal of Biological Control, 2004, 20(1): 76-78.
Bagheri N, Ahmadzadeh M, Heydari R. Effects of Pseudomonas fluorescens strain UTPF5 on the mobility, mortality and hatching of root-knot nematode Meloidogyne javanica. Archives of Phytopathology and Plant Protection, 2014, 47(6): 744-752.
Norabadi M T, Sahebani N, Etebarian H R. Biological control of root-knot nematode (Meloidogyne javanica) disease by Pseudomonas fluorescens (Chao). Archives of Phytopathology and Plant Protection, 2014, 47(5): 615-621.
Lingaiah S, Umesha S. Pseudomonas fluorescens inhibits the Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen in rice. Canadian Journal of Plant Protection, 2013, 1(5): 147-153.
Agarwal S. Efficacy of Pseudomonas fluorescens and Bacillus subtilis against Fusarium oxysporum f sp capsici and Meloidogyne incognita of chilli. Xian: Sam Higginbottom Institute of Agriculture, Technology and Sciences, 2013.
Soesanto L, Mugiastuti E, Rahayuniati A R F, et al. Efficacy of Pseudomonas fluorescens P60 in organic liquid for suppresion of Sclerotium rolfsii in cucumber. Recent advances in biofertilizers and biofungicides (PGPR) for sustainable agriculture. Proceedings of 3rd Asian Conference on Plant Growth-Promoting Rhizobacteria (PGPR) and other Microbials, Manila, Philippines, 21-24 April, 2013. Asian PGPR Society for Sustainable Agriculture, 2013: 393-415.
Hua J, Li L L, Cheng H, et al. Isolation, identification and screening of antagonistic bacteria of the pathogenic fungi in Chinese chestnut from Luotian county. Chinese Agricultural Science Bulletin, 2013, 29(25): 102-107.
Jiang J Z, Liang T Y, Wang H Y, et al. Screening of antagonistic Pseudomonas fluorescens against Phytophthora infestans and disease control in vitro. Journal of Agricultural University of Hebei, 2013, 36(3): 72-76.
Zegeye E D, Santhanam A, Gorfu D, et al. Biological activity of Trichoderma viride and Pseudomonas fluorescens against Phytophthora infestans under greenhouse condition. Journal of Agricultural Technology, 2011, 7(6): 1589-1602.
Li H, Li H B, Bai Y, et al. The Use of Pseudomonas fluorescens P13 to control sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape. The Journal of Microbiology, 2011, 49(6): 884-889.
Etebarian H R, Sholberg P L, Eastwell K C, et al. Biological control of apple blue mold with Pseudomonas fluorescens. Canadian Journal of Microbiology, 2005, 51(7): 591-598.
Wang G, Yang Z W. Inhibitory action of Pseudomonas fluorescens P2-5 strain to Gaeumannomyces graminis var. tritici. Plant Protection, 2004, 30(4): 32-34.
González-Sánchez M , de Vicente A, Pérez-García A, et al. Evaluation of the effectiveness of biocontrol bacteria against avocado white root rot occurring under commercial greenhouse plant production conditions. Biological Control, 2013, 67(2): 94-100.
Sang M K, Shrestha A, Kim D Y, et al. Biocontrol of phytophthora blight and anthracnose in pepper by sequentially selected antagonistic rhizobacteria against phytophthora capsici. The Plant Pathology Journal, 2013, 29(2): 154-167.
Liu J L, Fang F, Shi X H, et al. Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil. Acta Prataculturae Sinica, 2013, 22(2): 132-139.
Xi Q L, Yao T, Zhang D G. Effect of associative nitrogen fixation bacteria on Avena sativa growth. Acta Prataculturae Sinica, 2007, 16(3): 38-42.
Yao T, Yasmin S, Hafeez F Y. Potential role of rhizobacteria isolated from Northwestern China for enhancing wheat and oat yield. The Journal of Agricultural Science, 2008, 146: 49-56.
Yao T, Long R J, Zhang D G, et al. Isolation and characterization of plant growth promoting diazotrophs from the rhizosphere of wheat growing in saline soils in Northwestern China. 57th Annual conference of Canadian Society of Microbiologists. June 17-20, 2007.
Yao T. Research progress of plant growth promoting Rhizobacteria. Grassland and Turf, 2002, (4): 3-5.
Han H W, Sun L N, Yao T, et al. Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa. Acta Prataculturae Sinica, 2013, 22(5): 104-112.
Bi J T, Ma P, Yang Z W, et al. Isolation of endophytic fungi from the medicinal plant Tamarix chinensis and their microbial inhibition activity. Acta Prataculturae Sinica, 2013, 22(3): 132-138.
Hol W H, Bezemer T M, Biere A. Getting the ecology into interactions between plants and the plant growth-promoting bacterium Pseudomonas fluorescens. Frontiers in Plant Science, 2013, 4(81): 1-9.
Pan J, Mao Z S, Li X, et al. Preliminary study on pomegranate wilt management by employing Bacillus subtilis and Pseudomonas florescence isolates. Journal of Yunnan Agricultural University, 2013, 28(1): 27-31.
Mao Z S, Ma Q Y, Huang Q, et al. Antagonism of different mix proportions of bacteria strains to Fusarium solani. Journal of Yunnan Agricultural University, 2005, 20(1): 20-22.
Marimuthu S, Ramamoorthy V, Samiyappan R, et al. Intercropping system with combined application of azospirillum and Pseudomonas fluorescens reduces root rot incidence caused by Rhizoctonia bataticola and increases seed cotton yield. Journal of Phytopathology, 2013, 161(6): 405-411.
Mukherjee A, Sinha B S. Pseudomonas fluorescens mediated suppression of Meloidogyne incognita infection of cowpea and tomato. Archives of Phytopathology and Plant Protection, 2013, 46(5): 607-616.
Ran L X, Xiang M L, Li Z N, et al. Control of Eucalypt bacterial wilt by combination of two different suppression mechanisms. Journal of Central South University of Forestry & Technology, 2008, 28(4): 87-91.
Niu S G, Jiang S R, Tang W H. Positive regulations of Pseudomonas fluorescens by carbendazim and its application in controlling cotton Verticillium wilt. Acta Phytophylacica Sinica, 1999, 26(2): 171-176.
Zhang W Q, Nie M, Wang H, et al. Effect of metalions on cell growth and antibiotic production of Pseudomonas fluorescens P13. Journal of Shanghai Normal University (Natural Sciences), 2007, 36(2): 77-81.
Li X M, Hu B S, Xu Z G, et al. Interaction among antagonistic Bacillus spp. strains and their suppression against rice sheath blight. Acta Phytophylacica Sinica, 2003, 30(3): 273-278.
Haas D, Keel C.Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annual Review of Phytopathology, 2003, 41: 117-153.
Fischer S, Príncipe A, Alvarez F, et al. Fighting Plant Diseases Through the Application of Bacillus and Pseudomonas Strains. Symbiotic Endophytes. Springer Berlin Heidelberg, 2013: 165-193.
Nagel K, Schneemann I, Kajahn I, et al. Beneficial effects of 2, 4-diacetylphloroglucinol-producing pseudomonads on the marine alga Saccharina latissima. Aquatic Microbial Ecology, 2012, 67(3): 239.
Maurhofer M, Baehler E, Notz R, et al. Cross talk between 2, 4-diacetylphloroglucinol-producing biocontrol pseudomonads on wheat roots. Applied and Environmental Microbiology, 2004, 70(4): 1990-1998.
Vanitha S, Ramjegathesh R. Bio control potential of Pseudomonas fluorescens against coleus root rot disease. Plant Pathology Microbiology, 2014, 216(5): 2.
Jamali F, Sharifi-Tehrani A, Lutz M P, et al. Influence of host plant genotype, presence of a pathogen, and coinoculation with Pseudomonas fluorescens strains on the rhizosphere expression of hydrogen cyanide- and 2, 4-diacetylphloroglucinol biosynthetic genes in P. fluorescens biocontrol strain CHA0. Microbial Ecology, 2009, 57(2): 267-275.
Sajeli B A, Basha S A, Raghavendra G, et al. Isolation and characterization of antimicrobial cyclic dipeptides from Pseudomonas fluorescens and their efficacy on sorghum grain mold fungi. Chemistry & Biodiversity, 2014, 11(1): 92-100.
Yang M M, Wen S S, Mavrodi D V, et al. Biological control of wheat root diseases by the CLP-producing strain Pseudomonas fluorescens HC1-07. Phytopathology, 2014, 104(3): 248-256.
Souza J T. Distribution Diversity and Activity of Antibiotic-producing Pseudomonas spp. The Netherland Wageningen University, 2002.
Tran H, Ficke A, Asiimwe T, et al. Role of the cyclic lipopeptide massetolide A in biological control of Phytophthpra infestans and in colonization of tomato plants by Pseudomonas fluorescens. New Phytologist, 2007, 175(4): 731-742.
Li J, An D R, Li A R, et al. Isolation, purification, and structural characterization of antibiotic substance from Pseudomonas fluorescens AbIII745-6. Journal of Microbiology, 2008, 28(2): 11-14.
Asadhi S, Bhaskara R B, Sivaprasad Y, et al. Characterisation, genetic diversity and antagonistic potential of 2, 4-diacetylphloroglucinol producing Pseudomonas fluorescens isolates in groundnut-based cropping systems of Andhra Pradesh, India. Archives of Phytopathology and Plant Protection, 2013, 46(16): 1966-1977.
Arseneault T, Goyer C, Filion M. Phenazine production by Pseudomonas sp. LBUM223 contributes to the biological control of potato common scab. Phytopathology, 2013, 103(10): 995-1000.
Ran L X, Xiang M L, Zhou B. Siderophores are the main determinants of Fluorescent Pseudomonas strains in suppression of grey mould in Eucalyptus urophylla. Acta Phytopathologica Sinica, 2005, 35(1): 6-12.
Wei H L, Zhou H Y, Zhang L Q, et al. Experimental evidence on the functional agent of 2, 4-diacetylphloroglucinol in biocontrol activity of Pseudomonas fluorescens 2P24. Acta Microbiologica Sinica, 2004, 44(5): 663-666.
Lagzian A, Saberi R R, Khodaygan P, et al. Biocontrol performance evaluation of spontaneous mutants of Pseudomonas fluorescens VUPf5 generated during proliferation. Archives of Phytopathology and Plant Protection, 2013, 46(17): 2087-2095.
Kidarsa T A, Shaffer B T, Goebel N C, et al. Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS. Environmental Microbiology, 2013, 15(3): 716-735.
Liu J C, Zhang W, Wu X G, et al. Effcet of retS gene on biosynthesis of 2, 4-diacetylphloroglucinol in Pseudomonas fluorescens 2P24. Acta Microbiologica Sinica, 2013, 53(2): 118-126.
Bottiglieri M, Keel C. Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2, 4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0. Applied and Environmental Microbiology, 2006, 72(1): 418-427.
Lugtenberg B, Girard G. Role of Phenazine-1-Carboxamide Produced by Pseudomonas chlororaphis PCL1391 in the Control of Tomato Foot and Root Rot. Microbial Phenazines. Springer Berlin Heidelberg, 2013: 163-175.
Perneel M, Heyrman J, Adiobo A, et al. Characterization of CMR5c and CMR12a, novel fluorescent Pseudomonas strains from the cocoyam rhizosphere with biocontrol activity. Journal of Applied Microbiology, 2007, 103(4): 1007-1020.
Yao T. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine regionⅡPhosphate-solubilizing power and auxin production. Acta Prataculturae Sinica, 2004, 13(3): 85-90.
Yao T, Pu X P, Zhang D G, et al. Associative nitrogen- fixing bacteria in the rhizosphere of Avena sativa in an alpine region Ⅲ Effect on Avena sativa growth and quantification of nitrogen fixed. Acta Prataculturae Sinica, 2004, 13(5): 101-105.
Yao T, Long R J, Wang G, et al. Isolation and characteristics of associative symbiotic nitrogen bacteria from rhizosphere of wheat in saline soil in Lanzhou area. Acta Pedologica Sinica, 2004, 41(3): 444-448.
Yao T, Wang G, Chen B J, et al. Enumeration of associative symbiotic nitrogen fixation bacteria in the rhizoshpere of wheat in saline soil. Chinese Journal of Soil Science, 2004, 35(4): 479-482.
Yao T, Zhang D G, Long R J, et al. Study on associative nitrogen bacteria in rhizosphere of oat in alpine region. In Biological Nitrogen Fixation Sustainable Agriculture and the Environment. Proceedings of 14th International Nitrogen Fixation Congress, Springer. the Netherlands, 2005.
Yao T. Research progress on associative nitrogen fixation (ANF) within Gramineae rhizospheres. Chinese Grass Crops Pathology. Beijing: Ocean Press, 2003: 231-239.
Dubuis C, Keel C, Haas D. Dialogues of root-colonizing biocontrol pseudomonads. New Perspectives and Approaches in Plant Growth-Promoting Rhizobacteria Research. Springer Netherlands, 2007: 311-328.
Chi S Y, He Y Q, Han Z M, et al. Studies on complex infecting of pinewood nematodes and the bacterium carried by it. Journal of Fujian College of Forestry, 2008, 28(1): 92-96.
Wang R. The Identification on the Secondary Invader and Agent of Tobacco Physiological Disease. Fujian: Fujian Agriculture and Forestry University, 2013.
Xu Z W, Li X Y, Yang X S, et al. Analysis of spoilage ability of donimant spoilage bacterial from stored chilled Cyprinus carpio and Oreochromis niloticus. Food Science, 2012, 33(4): 243-246.
Du Z J, Huang X L, Deng Y Q, et al. Isolation and identification of Pseudomonas fluorescens from Andrias davidianus. Journal of Sichuan Agricultural University, 2011, 29(1): 103-107.
Deng X W, Xie Z X, Liu J B, et al. Isolation and identification of Pseudomonas fluorescens in tilapia. Guangxi Agricultural Sciences, 2010, 41(6): 612-615.
Chen L P, Hou F J, Zhang D J, et al. Distribution characteristics of Pseudomonas in Ningbo coastal sewage outlets. Oceanologia Et Limnologia Sinica, 2013, 44(4): 926-930.
黄云, 张中义, 黄丽丽, 等. 植物病害的定义刍议、利弊分析及综合控制. 植物保护, 2009, 35(1): 97-101.
严婉荣, 赵廷昌, 肖彤斌, 等. 生防细菌在植物病害防治中的应用. 基因组学与应用生物学, 2013, 32(4): 533-539.
刘琴, 刘翼, 何月秋, 等. 我国植物病害生物防治综述. 安徽农学通报, 2012, 18(7): 67-69.
何礼远. 细菌在植物病害生物防治上应用研究的进展. 中国生物防治, 1985, 1(3): 28-31.
马成涛, 胡青, 杨德奎. 土壤有益微生物防治植物病害的研究进展. 山东科学, 2007, 20(6): 61-67.
布坎南R E, 吉本斯N E. 伯杰细菌鉴定手册(第八版). 北京: 科学出版社, 1984.
东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 北京: 科学出版社, 2002: 162-166.
谭周进, 肖罗, 谢丙炎, 等. 假单胞菌的微生态调节作用. 核农学报, 2004, 18(1): 72-76.
杨毅, 李治, 高玲霞, 等. 荧光假单胞菌抗生性代谢产物合成相关基因的研究现状. 中国生物工程杂志, 2012, 32(8): 100-106.
王平, 李慧, 邱译萱, 等. 荧光假单胞菌株P13分泌铁载体抑制油菜菌核病菌. 上海师范大学学报(自然科学版), 2010, 39(2): 200-203.
袁理利. 产PCA基因工程菌M18Q发酵条件优化. 上海: 上海交通大学, 2008.
张伟琼, 聂明, 肖明. 荧光假单胞菌生防机理的研究进展. 生物学杂志, 2007, 24(3): 9-11, 24.
杨海君, 谭周进, 肖启明, 等. 假单胞菌的生物防治作用研究. 中国生态农业学报, 2004, 12(3): 158-161.
吕祝邦, 李敏权, 惠娜娜, 等. 甘肃省定西市当归"水烂病"病原鉴定及致病性测定. 植物保护, 2013, 39(2): 45-49.
李怀波, 彭珺, 包衍, 等. 拮抗油菜菌核病菌的荧光假单胞菌的分离与筛选. 中国农学通报, 2005, 21(11): 334-337.
张鹏, 李莉, 王智学, 等. 抗番茄灰霉病优良菌株的筛选与鉴定. 微生物学杂志, 2012, 32(6): 42-46.
董国菊, 李文英, 刘翠平, 等. 烟草疫霉拮抗菌株P-72-10的鉴定及其拮抗代谢产物初步分析. 植物病理学报, 2012, 42(3): 297-305.
陈思宇, 陈志谊, 张荣胜. 水稻纹枯病菌拮抗细菌的筛选及鉴定. 植物保护学报, 2013, 40(3): 211-218.
汪雪静, 卜春亚, 靳永胜, 等. 草莓根腐病菌拮抗细菌的分离与鉴定. 园艺学报, 2011, 38(9): 1657-1666.
李洪林. 荧光假单胞菌生防菌株的筛选及对烟草赤星病菌的抑制作用. 黑龙江: 黑龙江八一农垦大学, 2008.
李爱荣, 安德荣. 两株生防荧光假单胞杆菌的室内筛选试验. 微生物学杂志, 2003, 23(4): 11-13.
汤春梅, 陈秀蓉, 姚拓, 等. 九种根际促生菌最适培养条件初探. 草原与草坪, 2005, (3): 27-30.
昝继清, 林炜铁. 一株絮凝剂产生菌的筛选鉴定及培养条件. 微生物学通报, 2010, 37(4): 547-552.
赵淑莉, 任飞娥, 刘金亮, 等. 玉米大斑病生防放线菌的筛选鉴定及发酵条件优化. 微生物学报, 2012, 52(10): 1228-1236.
刘娜, 王琨. 生姜中抗黑曲霉活性内生细菌的筛选与鉴定. 中国农学通报, 2013, 29(4): 57-61.
万秀清, 郭兆奎, 颜培强, 等. 烟草炭疽病拮抗生防菌的筛选. 微生物学通报, 2008, 35(11): 1727-1731.
荣良燕, 姚拓, 赵桂琴, 等. 产铁载体PGPR菌筛选及其对病原菌的拮抗作用. 植物保护, 2011, 37(1): 59-64.
乔婵, 陈星, 张艳菊, 等. PF7-5对烟草角斑病的室内及大田防治效果. 中国林副特产, 2010, 108(5): 18-20.
李洪林, 万秀清, 颜培强, 等. 荧光假单胞杆菌G20-9拮抗烟草赤星病菌研究. 烟草科技, 2008, 249(4): 56-59.
顾金刚, 方敦煌, 李天飞, 等. 两株荧光假单胞菌菌株对烟草黑胫病病原菌的抑制作用. 中国生物防治, 2004, 20(1): 76-78.
华娟, 李淋玲, 程华, 等. 罗田栗实致腐真菌的分离鉴定及拮抗菌筛选. 中国农学通报, 2013, 29(25): 102-107.
蒋继志, 梁廷银, 王怀远, 等. 致病疫霉拮抗菌荧光假单胞菌的筛选及离体防病作用. 河北农业大学学报, 2013, 36(3): 72-76.
王刚, 杨之为. 荧光假单胞菌P2-5菌株对小麦全蚀病的抑制作用. 植物保护, 2004, 30(4): 32-34.
刘佳莉, 方芳, 史煦涵, 等. 2株盐碱地燕麦根际促生菌的筛选及其促生作用研究. 草业学报, 2013, 22(2): 132-139.
席琳乔, 姚拓, 张德罡. 固氮菌对燕麦不同生育期促生作用的研究. 草业学报, 2007, 16(3): 38-42.
姚拓. 促进植物生长菌的研究进展. 草原与草坪, 2002, (4): 3-5.
韩华雯, 孙丽娜, 姚拓, 等. 不同促生菌株组合对紫花苜蓿产量和品质的影响. 草业学报, 2013, 22(5): 104-112.
毕江涛, 马萍, 杨志伟, 等. 药用植物柽柳内生真菌分离及其抑菌活性初步研究. 草业学报, 2013, 22(3): 132-138.
潘俊, 毛忠顺, 李霞, 等. 利用枯草芽孢杆菌和荧光假单胞菌防治石榴枯萎病的初步研究. 云南农业大学学报, 2013, 28(1): 27-31.
毛忠顺, 马青云, 黄琼, 等. 细菌分离物不同比例混合对镰刀菌的拮抗作用. 云南农业大学学报, 2005, 20(1): 20-22.
冉隆贤, 向妙莲, 李正楠, 等. 将两种抗病机制结合控制桉树青枯病的研究. 中南林业科技大学学报(自然科学版), 2008, 28(4): 87-91.
牛赡光, 江树人, 唐文华. 多菌灵对荧光假单胞菌的正向调控作用及其应用. 植物保护学报, 1999, 26(2): 171-176.
张伟琼, 聂明, 王慧, 等. 金属离子对荧光单假胞菌P13生长及抗真菌素合成的影响. 上海师范大学学报(自然科学版), 2007, 36(2): 77-81.
李湘民, 胡白石, 许志刚, 等. 拮抗细菌菌株Bacillus spp.间的相互作用及其对水稻纹枯病的抑制. 植物保护学报, 2003, 30(3): 273-278.
李晶, 安德荣, 李爱荣, 等. 荧光假单胞杆菌AbIII745-6抑菌物质分离纯化和结构鉴定. 微生物学杂志, 2008, 28(2): 11-14.
冉隆贤, 向妙莲, 周斌. 荧光假单胞杆菌的嗜铁素是控制桉树灰霉病的主要因子. 植物病理学报, 2005, 35(1): 6-12.
魏海雷, 周洪友, 张力群, 等. 抗生素2, 4-二乙酰基间苯三酚作为荧光假单胞菌2P24菌株生防功能因子的实证分析. 微生物学报, 2004, 44(5): 663-666.
刘九成, 张伟, 吴小刚, 等. 荧光假单胞菌2P24中retS对抗生素2,4-二乙酰基间苯三酚合成的影响. 微生物学报, 2013, 53(2): 118-126.
姚拓. 高寒地区燕麦根际联合固氮菌研究Ⅱ固氮菌的溶磷性和分泌植物生长素特性测定. 草业学报, 2004, 13(3): 85-90.
姚拓, 蒲小鹏, 张德罡, 等. 高寒地区燕麦根际联合固氮菌研究Ⅲ固氮菌对燕麦生长的影响及其固氮量测定. 草业学报, 2004, 13(5): 101-105.
姚拓, 龙瑞军, 王刚, 等. 兰州地区盐碱地小麦根际联合固氮菌分离及部分特性研究. 土壤学报, 2004, 41(3): 444-448.
姚拓, 王刚, 陈本建, 等. 盐碱地小麦根际联合固氮菌数量分布特征研究. 土壤通报, 2004, 35(4): 479-482.
姚拓. 禾本科植物根际联合固氮资源研究进展. 中国草类作物病理学研究. 北京: 海洋出版社, 2003: 231-239.
池树友, 何月秋, 韩正敏, 等. 松材线虫及携带细菌对黑松复合的侵染. 福建林学院学报, 2008, 28(1): 92-96.
王蕊. 烟草生理性病害病因及其次生侵染菌鉴定. 福建: 福建农林大学, 2013.
许振伟, 李学英, 杨宪时, 等. 冷藏鲤鱼和罗非鱼优势腐败菌腐败能力分析. 食品科学, 2012, 33(4): 243-246.
杜宗君, 黄小丽, 邓永强, 等. 人工养殖大鲵病原荧光假单胞菌的分离鉴定. 四川农业大学学报, 2011, 29(1): 103-107.
邓显文, 谢芝勋, 刘加波, 等. 罗非鱼荧光假单胞菌的分离鉴定. 广西农业科学, 2010, 41(6): 612-615.
陈丽萍, 侯付景, 张迪骏, 等. 宁波沿海陆源排污口假单胞菌属. 海洋与湖沼, 2013, 44(4): 926-930.