Genetic variation of Dactylis glomerata germplasm from Southwest China detected by SSR markers

Abstract

Abstract: Simple sequence repeat (SSR) molecular markers were applied to detect the genetic variation of 110 individuals of 11 Dactylis glomerata accessions from Southwest China(Sichuan province, Yunnan province,Guizhou province and Chongqing city). 1) 21 primer pairs were selected for SSR analysis and 143 polymorphic bands were found with an average of 6.8 bands per primer pair. The percentage of polymorphism bands (PPB) was 90.7%, the polymorphism information content (PIC) ranged from 0.17(A01F24) to 0.45(A01E02) with an average of 0.33, and the Shannon’s information index of diversity (I) ranged from 0.046 3 to 0.347 7, showing considerable genetic diversity between all the accessions. 2) The AMOVA analysis showed considerable genetic variation within accessions (63.39%) rather than between them (21.93%). There was no significant genetic variation between diploid and tetraploid groups. 3) UPGMA clusters based on average Nei’s genetic identity divided 11 accessions into two groups: 3 diploid accessions (YA2006-3, YA2006-4, YA02-101) collected from Chongqing and Guizhou were classified into group I and the remaining 8 accessions were classified into group II. In most cases, materials from the same region and ploidy level were in the same group thus showing a geographical distribution of genetic diversity of the tested materials.

CLC Number:

XIE Wen-gang, ZHANG Xin-quan, MA Xiao, HUANG Lin-kai, ZENG Bing. Genetic variation of Dactylis glomerata germplasm from Southwest China detected by SSR markers[J]. Acta Prataculturae Sinica, 2009, 18(4): 138-146.

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