Analysis of genetic diversity in a cocksfoot (Dactylis glomerata) variety using SCoT markers

doi:10.11686/cyxb20140128

Abstract

Abstract: Start codon targeted polymorphism (SCoT) was used to analysis 32 cocksfoot (Dactylis glomerata) varieties (including cultivated and introduced varieties),to reveal the differences of genetic diversity in cultivated and introduced varieties and to provide a theoretical basis for breeding new varieties of cocksfoot. 1) Twenty-two primers were selected from 80 SCoT primers,and a total of 308 bands were amplified,of which 245 (79.55%) were polymorphic: the average was 11.14 per primer. 2) The average genetic distance of cultivated varieties was 0.1242 while that of introduced varieties was 0.1952. The introduced varieties had higher genetic distances than the cultivated ones at the level of polymorphism bands (NPB) and percentage of polymorphic bands (PPB). The gene diversity index (H) and Shannon index (I),indicated that the genetic diversity of cultivated varieties was lower than that of introduced varieties. 3) AMOVA analysis showed that the genetic variance within and between cultivated and introduced varieties was 83.68% and 16.32%,respectively and the main genetic variance was in the internal group. 4) The results of UPGMA clustering and PCoA analysis showed that the 32 cocksfoot varieties could be divided into six groups,the cultivated varieties were in group I while the introduced varieties were in the other five groups.

CLC Number:

S543⁺.303

JIANG Lin-feng,ZHANG Xin-quan,HUANG Lin-kai,MA Xiao,YAN De-fei,HU Qiang,FU Yu-feng. Analysis of genetic diversity in a cocksfoot (Dactylis glomerata) variety using SCoT markers[J]. Acta Prataculturae Sinica, 2014, 23(1): 229-238.

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