Phylogeny of species with the StH genome in Triticeae (Poaceae) inferredfrom nuclear rDNA ITS sequences

Abstract

Abstract: Species with the StH genome in Triticeae are included in the genus Elymus sensu lato. In order to investigate the phylogeny of the StH genome in Triticeae, nuclear ribosomal internal transcribed spacer (ITS) sequences of 16 species with the StH genome were analyzed together with those of five Pseudoroegneria (St) and three Hordeum (H) accessions. The maximum marsimony (MP), maximum likelihood (ML) and median joining algorithm (MJ) methods were used to reconstruct the phylogenetic tree. The results indicated that:1) Sitanion jubatum, Sitanion hystrix and Hystrix patula are closely related to Elymus species, and should be transferred to Elymus; 2) the StH genome species distributed in North American have close relationships. There is diversity and differentiation between the North American and Eurasian StH genome species; 3) a certain degree of diversity and differentiation of StH genome species is in good agreement with their geographical distributions;4) a certain degree of differentiation was found in diploid species with St and H genomes. Meanwhile, the possible reasons for the differentiation of StH genome species are also discussed.

CLC Number:

WANG Xiao-li, FAN Xing,ZHANG Chun, SHA Li-na,
ZHANG Hai-qin, ZHOU Yong-hong. Phylogeny of species with the StH genome in Triticeae (Poaceae) inferredfrom nuclear rDNA ITS sequences[J]. Acta Prataculturae Sinica, 2009, 18(6): 82-90.

References

[1] Lve . Conspectus of the Triticeae[J]. Feddes Repertorium, 1984, 95: 425-521.
[2] Dewey D R. The genome system of classification as a guide to intergeneric hybridization with the perennial Triticeae[A]. In: Gustafson J P. Gene Manipulation in Plant Improvement[M]. New York: Plenum Press, 1984. 209-279.
[3] Jiang J M, Friebe B, Gill B S. Recent advances in alien gene transfer in wheat[J]. Euphytica, 1994, 73: 199-212.
[4] 万里强, 石永红, 李向林, 等. 高温干旱胁迫下三个多年生黑麦草品种叶绿体和线粒体超微结构的变化[J]. 草业学报, 2009, 18(1): 25-31.
[5] 董全民, 赵新全, 马有泉, 等. 垂穗披碱草/星星草混播草地优化牦牛放牧强度的研究[J]. 草业学报, 2008, 17(5): 16-22.
[6] 张耿, 王赞, 高洪文, 等. 21份偃麦草属牧草苗期耐盐性评价[J].草业科学, 2008, (1): 51-54.
[7] Tzvelev N N. The system of grass (Poaceae) and their evolution[J]. The Botanical Review, 1989, 55: 141-204.
[8] Moench C. Methodus Plantas Horti Botanici et Agri Marburgensis a Staminum Situ Describendi[M]. Margburgi Cattorum, 1794.
[9] Wilson F D. Revision of Sitanion (Triticeae, Grameneae)[J]. Brittonia, 1963, 15: 303-323.
[10] 耿以礼. 中国主要植物图说——禾本科[M]. 北京: 科学出版社, 1959. 342-409.
[11] Sun G L, Ni Y, Daley T. Molecular phylogeny of RPB2 gene reveals multiple origin, geographic differentiation of H genome, and the relationship of the Y genome to other genomes in Elymus species[J]. Molecular Phylogenetics and Evolution, 2008, 46: 897-907.
[12] Yu H Q, Fan X, Zhang C, et al. Phylogenetic relationships of species in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from nuclear rDNA ITS (internal transcribed spacer) sequences[J]. Biologia, 2008, 63: 498-505.
[13] Hsiao C, Chatterton N J, Asay K H, et al. Phylogenetic relationships of the monogenomic species of the wheats tribe, Triticeae (Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences[J]. Genome, 1995, 38: 221-223.
[14] 严学兵, 王堃, 周禾, 等.不同来源SSR标记在我国披碱草属植物的通用性和效率评价[J]. 草业学报, 2008, 17(6): 112-120.
[15] Liu Q L, Ge S, Tang H B, et al. Phylogenetic relationships in Elymus (Poaceae: Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences[J]. New Phytologist, 2006, 170: 411-420.
[16] Doyle J J, Doyle J L. Isolation of plant DNA from fresh tissue[J]. Focus, 1990, 12: 13-15.
[17] Thompson J D, Higgins D G, Gibson T J. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research, 1994, 22: 4673-4680.
[18] Swofford D L. PAUP^*: Phylogenetic Analysis Using Parsimony (^*and other method)(Version 4.0)[M/OL]. Sinauer Associates, Sunderland-Massachusetts, http: //www. sinauer. com, 2003.
[19] Posada D, Crandall K A. Modeltest: Testing the model of DNA substitution[J]. Bioinformatics, 1998, 14: 817-818.
[20] Bandelt H J, Forster P, Rohl A. Median-joining networks for inferring intraspecific phylogenies[J]. Molecular Biology and Evolution, 1999, 16: 37-48.
[21] Zhang H Q, Yang R W, Dou Q W, et al. Genome constitutions of Hystrix patula, H. duthiei ssp. duthiei and H. duthiei ssp. longearistata (Poaceae: Triticeae) revealed by meiotic pairing behavior and genomic in-situ hybridization[J]. Chromosome Research, 2006, 14: 595-604.
[22] Jensen K B, Wang R R-C. Cytological and molecular evidence for transferring Elymus coreanus and Elymus californicus from the genus Elymus to Leymus (Poaceae: Triticeae)[J]. International Journal of Plant Science, 1997, 158: 872-877.
[23] Dewey D R. A cytogenetic study of Agropyron stipifolium and its hybrids with Agropyon repens[J]. Bulletin of the Torrey Botanical Club, 1970, 97: 315-320.
[24] rgaard M, Anamthawat-Jónsson K. Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae)[J]. Genome, 2001, 44: 275-283.
[25] Mason-Gamer R J. Reticulate evolution, introgression, and intertribal gene capture in an allohexaploid grass[J]. Systematic Biology, 2004, 53: 25-37.
[26] Jakob S S, Blattner F R. A chloroplast genealogy of Hordeum (Poaceae): Long-term persisting haplotypes, incomplete lineage sorting, regional extinction, and the consequences for phylogenetic inference[J]. Molecular Biology and Evolution, 2006, 23: 1602-1612.
[27] Kilian B, zkan H, Deusch O, et al. Independent wheat B and G genome origins in outcrossing Aegilops progenitor haplotypes[J]. Molecular Biology and Evolution, 2007, 24: 217-227.
[28] Cassens I, Mardulyn P, Milinkovitch M C. Evaluating intraspecific “Network” construction methods using simulated sequence data: Do existing algorithms outperform the global maximum parsimony approach?[J]. Systematic Biology, 2005, 54(3): 363-372.
[29] Jaaska V. Isoenzyme variation in the grass genus Elymus (Poaceae)[J]. Hereditas, 1992, 117: 11-22.
[30] Sun G L, Salomon B, Bothmer R von. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers[J]. Genome, 1997, 40: 806-814.
[31] Stebbins J L, Pun F T. Artificial and natural hybrids in the Gramineae, tribe Hordeae. V. Diploid hybrids of Agropyron[J]. American Journal of Botany, 1953, 40: 444-449.
[32] Bothmer R von, Baden C, Jorgensen R, et al. An ecogeographical study of the genus Hordeum[R]. International Board for Plant Genetic Resources (Food and Agriculture Organization), Rome, Italy, 1991.
[33] Linde-Laursen I, Seberg O, Salomon B. Comparison of the Giemsa C-banded and N-banded karyotypes of two Elymus species, E. dentatus and E. glaucescens (Poaceae: Triticeae)[J]. Plant Systematics and Evolution, 1994, 192: 165-176.
[34] Dubcovsky J, Schlatter A R, Echaide M. Genome analysis of South American Elymus (Triticeae) and Leymus (Triticeae) species based on variation in repeated nucleotide sequences[J]. Genome, 1997, 40: 505-520.