A study on cell suspension culture and flavonoids accumulation of Stellera chamaejasme

Abstract

Abstract: A cell suspension culture system was established using cultures of loose calli of Stellera chamaejasme to investigate the effects of different plant growth regulators on callus induction rate and growth state from explants such as buds, leaves and stem segments of S. chamaejasme, and to study the effects of carbon species and plant growth regulators on cell growth and flavonoids accumulation using orthogonal tests. The loose calli suitable for suspension culture can be induced from stem segments on MS with 2,4-D 1.0 mg/L+NAA 0.1 mg/L, and the induction rate can reach 90%. The effects of factors in the orthogonal test on cell growth and flavonoids content are consistent and in the order 2,4-D＞carbon source＞NAA＞6-BA. Higher concentrations of 2,4-D, NAA or 6-BA are more conducive to cell growth and flavonoids accumulation. Sucrose is most conducive to cell growth and flavonoids accumulation, followed by glucose and fructose. The optimum medium for cell growth and flavonoids accumulation is MS+2,4-D 2.0 mg/L+NAA 1.0 mg/L+6-BA 0.5 mg/L+surcrose 30 g/L. There is a significant positive correlation between cell growth and flavonoids content (R²=0.967, P<0.01), belonging to growth-coupling-type of the secondary metabolism model.

CLC Number:

WANG Wen-xing, AN Qi, WANG Ying, WANG Meng, CAO Cheng-you. A study on cell suspension culture and flavonoids accumulation of Stellera chamaejasme[J]. Acta Prataculturae Sinica, 2010, 19(6): 132-139.

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