The study of soil microbial biomass and soil enzyme avtivity on different grassland in Hulunbeier, Inner Mongolia

Abstract

Abstract: In this study, 5 different types of grassland (Filifolium sibiricum steppe, Stipa baicalensis steppe, Leymus chinensis steppe, Stipa grandis steppe, Stipa kryrowi steppe) in Hulunbeier were selected as the research object to analyze the variation of the soil physicochemical characteristics, soil microorganism, soil microbial biomass, soil enzyme activity and the inter-relationship among them. The results showed that the number of microorganisms in different types of grassland were in the same changing trends: bacteria>actinomycetes>fungi, but the number of each microorganism and composition reduced significantly (P<0.05). The bacteria number in 5 different types of glasslands was L. chinensis steppe>S. baicalensis steppe>S. kryrowi steppe>F. sibiricum steppe>S. grandis steppe, the fungi number was S. kryrowi steppe>L. chinensis steppe>F. sibiricum steppe>S. baicalensis steppe>S. grandis steppe, the actinomycetes number was F. sibiricum steppe>S. kryrowi steppe>L. chinensis steppe>S. baicalensis steppe>S. grandis steppe. Soil microbial biomass C, N (SMBC, SMBN) reduced significantly (P<0.05) in different grasslands. The microbial biomass carbon and nitrogen in F. sibiricum steppe soil were significantly higher than others (P<0.05), soil microbial biomass C and N in S. grandis steppe and L. chinensis steppe were lower. Results also showed that soil activitiey reduced significantly (P<0.05) in different grasslands with 0-10 cm higher than 0-20 cm in the vertical distribution. Soil activitoes of catalase and invertase activities of F. sibiricum steppe were significantliy higher than S. grandis steppe, S. baicalensis steppe; Soil urease activity of S. kryrowi steppe was the highest and significantly different from S. grandis steppe, F. sibiricum steppe, soil phosphatase activity in L. chinensis steppe was the highest and significantly different from others. Correlation analysis indicated that soil microbe quantity, soil microbial biomass, soil enzyme activity and soil physicochemical characteristics in different grasslands had different degrees of correlation. The regression formula indicated that soil microbial biomass C increased with soil moisture, bacteria quantity but decreased with soil phosphatase activity; soil microbial biomass N increased with soil hydrogen peroxidase activities, but decreased with soil phosphatase activity and soil bulk.

CLC Number:

WENDU Ri-le, LI Gang, ZHANG Jing-ni, LAI Xin, YI Jin, FAN Guo-yan, YANG Dian-lin. The study of soil microbial biomass and soil enzyme avtivity on different grassland in Hulunbeier, Inner Mongolia[J]. Acta Prataculturae Sinica, 2010, 19(5): 94-102.

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