Effect of exogenous metallothionein on blood GSH-Px and CAT gene expression in dairy cattle

Abstract

Abstract: The effect of exogenous metallothionein on gene expression of antioxidant enzymes in dairy cattle was conducted with 24 lactating Chinese Holstein cows, which were randomly divided into four groups (A, B, C, and D), and supplemented with 0, 4.0, 8.0, and 12.0 mg Zn-MT respectively by intravenous injection. Blood samples were collected every 15 days and the levels of GSH-Px and CAT gene expression in the cows were determined. The levels of GSH-Px gene expression of the cows in groups C and D, and the level of CAT gene expression in group C were greater (P<0.05) than those in group A. The level of CAT gene expression in group D was greater than those in groups A (P<0.01), B (P<0.05), and C (P>0.05), while there were no significant differences (P>0.05) in either GSH-Px and CAT gene expression levels between groups A and B. The levels of GSH-Px and CAT gene expression peaked at the 30th day and descended at the 45th day after the supply of exogenous metallothionein. The levels of GSH-Px gene expression at the 30th day and CAT gene expression at the 15th day and the 30th day after the supply of exogenous metallothionein were greater (P<0.05) than those before the supply. It is suggested that exogenous Zn-MT can promote the levels of GSH-Px and CAT gene expression of dairy cattle, showing a time-dependent and dose-dependent manner.

CLC Number:

S823

ZHANG Bin, TAN Qiong, LI Li-li, LIU Hai-lin, DE Xinghou, XIAO Ding-fu,CHEN Yu-guang, LUO Jia-jie, BI Xiao-yan. Effect of exogenous metallothionein on blood GSH-Px and CAT gene expression in dairy cattle[J]. Acta Prataculturae Sinica, 2010, 19(3): 132-138.

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