A study on the variance of Fusarium graminearum sampled from central Gansu

Abstract

Abstract: The occurrence of Fusarium graminearum on Triticum aestivum and Zea mays, in the middle areas of Gansu Province, China was assessed by isolation of Fusarium species from T. aestivum roots with brown rot symptoms, from T. aestivum sheaths, T. aestivum soil, Z. mays kernels, and Z. mays stalks. Fusarium isolates were identified by morphology according to Nelson’s system. Fourteen of 43 Fusarium isolates were F. graminearum. It was isolated only from corn stalks. These 14 isolates produced between 2 and 90 perithecia each. PCR using the Fg16 primer set resulted in a product of 0.41 kb from all 14 isolates and of 0.50 kb from the 6 reference isolates. It successfully differentiated between the F. graminearum isolates. PCR reactions using the Tri13 primer showed that all 14 isolates and the three Chinese reference cultures produced DON while the three Australian reference isolates produced fragments of 415 bp showing that they produced NIV.

CLC Number:

S435.121.4

LI Jin-hua, JI Li-jing, CHAI Zhao-xiang, Knight T E, Burgess L W. A study on the variance of Fusarium graminearum sampled from central Gansu[J]. Acta Prataculturae Sinica, 2009, 18(1): 118-124.

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