An analysis by ISSR of genetic diversities in Stylosanthes germplasms

Abstract

Abstract: Forty-eight Stylosanthes germplasms were studied for an analysis of genetic diversity by ISSR. Fourteen effective primers selected from 68 ISSR primers were used for PCR, and 155 of the 156 DNA fragments amplified, showed polymorphisms. The average binds from each primer was 11.1 and the average percentage of polymorphic bands was 99.36%. The coefficient of genetic conformability among the germplasms was 0.445-0.975. The results from POPGENE analysis indicated that the average information index (I) of Shannon was 0.355 3, gene diversity (H) of Nei was 0.227 9, and effective number of alleles (NE) was 1.388 7. Based on analyses of cluster and principal components, the 48 Stylosanthes germplasms used in this study could be divided into 5 groups: S. hamata, S. capitata, S. scabra, S. seabrana and S. guianensis, in which the genetic differentiation of S. guianensis group was the largest.

CLC Number:

S540.32
Q943

TANG Yan-qiong, HU Xin-wen, GUO Jian-chun, BAI Chang-jun, HE Hua-xuan. An analysis by ISSR of genetic diversities in Stylosanthes germplasms[J]. Acta Prataculturae Sinica, 2009, 18(1): 57-64.

References

[1] Williams R J, Reid R, Schultze-Kraft R, et al. Natural distribution of Stylosanthes[A]. In: Stace H M, Edye L A. The Biology and Agronomy of Stylosanthes[M]. Sydney, Australia: Academic Press, 1984.73-101.
[2] 蒋昌顺. 我国对柱花草的研究与利用[J]. 热带作物研究, 1995, (1): 61-65.
[3] Sousa Costa N M, Ferreira M B. Some Brazilian species of Stylosanthes[A]. In: Stace H M, Edye L A. The Biology and Agronomy of Stylosanthes[M].Sydney, Australia: Academic Press, 1984.23-48.
[4] Mannetje L T. Considerations on the taxonomy of the genus Stylosanthes[A]. In: Stace H M, Edye L A. The Biology and Agronomy of Stylosanthes[M]. Sydney, Australia: Academic Press, 1984.1-21.
[5] Glover B J, Gillies A C M, Abbott R J. Use of the polymerase chain reaction to investigate the delimitation of two agronomically important species of Stylosanthes (Aubl.) Sw[J]. Botanical Journal of Scotland, 1984, 47: 83-96.
[6] 蒋昌顺, 葛琴雅. 柱花草RAPD 反应体系的建立及其8个品种遗传多样性分析[J]. 广西植物, 2004, 24(3): 243-247.
[7] Curtis M D, Cameron D F, Manners J M. Molecular evidence that diploid Stylosanthes humilis and diploid Stylosanthes hamata are progenitors of allotetraploid Stylosanthes hamata cv ‘Verano’[J]. Genome, 1995, 38: 344-348.
[8] Jiang C S, Jia H S, Ma X R. AFLP analysis of genetic variability among Stylosanthes guianensis accessions resistant and susceptible to the Stylo[J]. Anthracnose Acta Botanica Sinica, 2004, 46(4): 480-488.
[9] Liu C J, Musial J M, Thomas D. Genetic relationship among Stylosanthes species revealed by RFLP and STS analysis[J]. Theoretical and Applied Genetics, 1999, 99: 1179-1186.
[10] Vander stappen J, Volckaert G. Molecular charaterization and classification of Stylosanthes mexicanna, S. macrocarpa, S. seabrana, and S. fruticosa by DNA sequence analysis of two chloroplast regions[J]. DNA Sequence, 1999, (3): 199-202.
[11] Vander stappen J, De Laet J, Gama-Lopez S. Phylogenetic analysis of Stylosanthes (Fabaceae) based on the internal transcribed spacer region (ITS) of nuclear ribosomal DNA[J]. Plant Systematics and Evolution, 2002, 234: 27-51.
[12] 蒋昌顺. 应用微卫星标记分析柱花草的遗传多样性[J]. 高技术通讯, 2004, (4): 25-30.
[13] Vander stappen J, Weltjens I, Volckaert G. Microsatellite markers in Stylosanthes guianensis[J]. Molecular Ecology, 1999, 8: 515-517.
[14] 蒋昌顺, 张新申. 柱花草核rDNA的ITSl序列分析[J]. 高技术通讯, 2006, (1): 73-77.
[15] Zietikiewicze, Rafalske R A, Labuda D. Genome fingerprinting by simple sequence repeat (ISSR) anchored and polymerase chain reaction amplificaion[J]. Genomica, 1994, 20: 178-183.
[16] Shen J, Ding X, Liu D, et al. Intersimple sequence repeats (ISSR) molecular fingerprinting markers for authenticating populations of Dendrobium officinale KIMURA et MIGO[J]. Biological and Pharmaceutical Bulletin, 2006, 29(3): 420-422.
[17] Amel S, Mikhtar T, Salwa Z, et al. Inter-simple sequence repeat fingerprints to assess genetic diversity in trnisian fig (Ficus carica L.) germplasm[J]. Genetic Resources and Crop Evolution, 2004, 51: 269-275.
[18] Ge X, Yu Y, Zhao N, et al. Genetic variation in the endangered inner mongolia endemic shrub Tetraena mongolica Mazim. (Zygophyllaceae)[J]. Biolgical Conservation, 2003, 111: 427-434.
[19] Wolfe A D, Xiang Q, Kephart S R. Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimple sequence repeat (ISSR) bands[J]. Milecular Ecology, 1998, 7: 1107-1125.
[20] Danilova T V, Danilov S S, Karlov G I. Assessment of genetic polmorphism in hop (Himulus lupulus L.) cultivars by ISSR-PCR analysis[J]. Russian Journal of Genetics, 2003, 39: 1252-1257.
[21] Pomper K W, Crabtree S B, Brown S P, et al. Assessment of genetic diversity of pawpaw (Asimina triloba) cultivators with intersimple sequence repeat markers[J]. Journal American Society for Horticultural Science, 2003, 128(4): 521-525.
[22] Talhinhas T, Neves-Martins J, Leitào J. AFLP, ISSR, and RAPD markers reveal high levels of genetic diversity among Lupinus ssp.[J]. Plant Breeding, 2003, 122: 507-510.
[23] 唐燕琼, 吴紫云, 郭建春, 等. 柱花草DNA提取及ISSR反应体系的正交优化[J]. 热带作物学报, 2008, 29(3): 352-357.
[24] 英彩, 赖志强, 腾少花, 等. 907柱花草的选育研究[J]. 草业科学, 1998, 15(2): 27-30.
[25] 刘国道, 白昌军, 何华玄, 等. 热研5号柱花草选育研究[J]. 草地学报, 2001, 9(1): 1-7.
[26] 范彦, 李芳, 张新全, 等. 扁穗牛鞭草种质遗传多样性的ISSR分析[J]. 草业学报, 2007, 16(4): 76-81.
[27] 刘伟, 张新全, 李芳, 等. 西南区野生狗牙根遗传多样性的ISSR标记与地理来源分析[J]. 草业学报, 2007, 16(3): 55-61.
[28] Prevost A, Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars[J]. Theoretical and Applied Genetics, 1999, 98: 107-112.
[29] Charters Y M, Robertson A, Wilkinson M J, et al. PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. olei fera) using 5′-anchored simple sequence repeat (SSR) primers[J]. Theoretical and Applied Genetics, 1996, 92: 442-447.
[30] 邓春婷, 包满珠. 野牛草实生群体多样性的表型及ISSR分析[J]. 草业科学, 2008, 25(1): 100-105.
[31] Huang J C, Sun M. Genetic diversity and relationships of sweptotato and its wild relatives in Jpomoea series Batats (Convolvlaceae) as revealed by ISSR and restriction analysis of chloroplast DNA[J]. Theoretical and Applied Genetics, 2000, 100: 1050-1060.
[32] 隋晓青, 王堃. 克氏针茅ISSR-PCR反应体系的建立与优化[J]. 草业学报, 2008, 17(3): 71-78.