A maize abscisic acid insensitive 3 gene confers drought and salt stress tolerance in Arabidopsis

doi:10.11686/cyxb2015330

Abstract

Abstract: The abscisic acid insensitive 3 gene (ABI3) has been widely studied in cereals such as wheat, maize and rice however, the functions of ABI3 have not been fully described. In this paper, a novel maize ABI3 like gene was cloned and named ZmABI3-L. This gene was predicted to encode a transcription factor with a distinct DNA-binding B3 domain. The full length of the gene was 1735 bp and with an opening read frame of 1212 bp and encoded 404 amino acids. Alignment of the ZmABI3-L proteins with other plants revealed similarities with ABI3 protein from other species. RT-PCR analysis showed ZmABI3-L was up-regulated in maize by dehydration, salt, cold and ABA stress. Over-expression of ZmABI3-L in Arabidopsis plants could enhance salt and drought stress tolerance compared to the wild type. The results suggest that ZmABI3-L may be involved in salt and drought resistance signaling pathways in maize.

YUAN Jin-Cheng, SONG Jin-Hui, MA Hai-Lian, WENG Qiao-Yun, WANG Ling-Yun, ZHAO Yan, LIU Ying-Hui. A maize abscisic acid insensitive 3 gene confers drought and salt stress tolerance in Arabidopsis[J]. Acta Prataculturae Sinica, 2016, 25(2): 124-131.

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