Cloning and expression of PtGAPDH from Puccinellia tenuiflora

doi:10.11686/cyxb20140225

Abstract

Abstract: A full length cDNA named PtGAPDH related to saline-alkali tolerance was cloned from Puccinellia tenuiflora by RT-PCR and RACE (rapid amplification of cDNA ends). PtGAPDH gene with 1014 bp open reading frame encoded a protein with a predicted length of 337 amino acids. Phylogenetic analysis indicates that this gene, denoted PtGAPDH, has sequence characteristics similar to other monocotyledon GAPDH genes. Northern blot shows the expression of PtGAPDH has increased with the growth of the concentration of Na₂CO₃ in the proper realm, otherwise, has decreased both in leaves and roots. The expression pattern of PtGAPDH gene stands for the regular effects of the saline-alkali stress on the gene expression of P. tenuiflora on the molecular standard.

CLC Number:

S540.3

ZHANG Yang, GUO Hai-jun, LIU Long-biao, WANG Sa, LIANG Ying, NIE Yu-zhe, LI Yu-hua. Cloning and expression of PtGAPDH from Puccinellia tenuiflora[J]. Acta Prataculturae Sinica, 2014, 23(2): 207-214.

References

[1] 孙国荣, 阎秀峰. 星星草生理生态学研究[M]. 北京: 科学出版社, 2000.
[2] 石德成, 殷立娟. 盐(NaCl)与碱(Na₂CO₃)对星星草胁迫作用的差异[J]. 植物学报, 1993, 35(2): 144-149.
[3] 刘桂丰, 褚延广, 王玉成, 等. 星星草cDNA文库构建和金属硫蛋白(MT-1)基因的克隆[J]. 植物生理学通讯, 2005, 41(4): 424-428.
[4] 刘桂丰, 褚延广, 王玉成, 等. cDNA微阵列技术研究NaHCO₃胁迫下星星草基因表达谱[J]. 西北植物学报, 2005, 25(5): 887-892.
[5] 李剑, 张金林, 王锁民, 等. 小花碱茅HKT2;1基因全长cDNA的克隆与生物信息学分析[J]. 草业学报, 2013, 22(2): 140-149.
[6] Danshina P V, Schmalhausen E V, Avetisyan A V, et al. Mildly oxidized glyceraldehydes-3-phosphate dehydrogenase as a possible regulator of glycolysis[J]. IUBMB Life, 2001, 51: 309-314.
[7] Sirover M A. Role of the glycolytic protein glyceraldehydes-3-phosphate dehydrogenase in normal cell function and in cell pathology[J]. Journal of Cellular Biochemistry, 1997, 66: 133-140.
[8] Sirover M A. New nuclear functions of the glycolytic protein glyceraldehydes-3-phosphate dehydrogenase in mammalian cells[J]. Journal of Cellular Biochemistry, 2005, 95: 45-52.
[9] Jurgensmeier J M, Krajewski S, Armstrong R C, et al. Bax-and Bak-induced cell death in the fission yeast Schizosaccharomyces pombe[J]. Molecular and Cellular Biology, 1997, 8: 325-339.
[10] Moon H, Baek D, Lee B, et al. Soybean ascorbate peroxidase suppresses Bax-induced apoptosis in yeast by inhibiting oxygen radical generation[J]. Biochemical and Biophysical Research Communications, 2002, 290: 457-462.
[11] Scheibe R, Backhausen J E, Emmerlich V, et al. Strategies to maintain redox homeostasis during photosynthesis under changing conditions[J]. Journal of Experimental Botany, 2005, 56: 1481-1489.
[12] Lindermayr C, Saalbach G, Durner J. Proteomic identification of S-nitrosylated proteins in Arabidopsis[J]. Plant Physiology, 2005, 137: 921-930.
[13] 杨洋, 张智俊, 罗淑萍. 毛竹甘油醛-3-磷酸脱氢酶基因的克隆与序列分析[J]. 经济林研究, 2010, 28(3): 7-13.
[14] 张乐新, 苏蔓, 马甜, 等. 羊草Δ1-吡咯琳-5-羧酸合成酶(LcP5CS1)基因的克隆与分析[J]. 草业学报, 2013, 22(4): 197-204.
[15] 李晓宇, 蔺吉祥, 李秀军, 等. 羊草苗期对盐碱胁迫的生长适应及Na⁺、K⁺代谢响应[J]. 草业学报, 2013, 22(1): 201-209.
[16] 尹尚军, 石德成, 颜宏. Na₂CO₃胁迫下星星草胁变反应与时间及胁强的关系[J]. 草业学报, 1999, 8(4): 46-50.
[17] Baek D, Jina Y, Jeong J C, et al. Suppression of reactive oxygen species by glycerald ehyde-3-phosphate dehydrogenase[J]. Phytochemistry, 2008, 69(2): 333-338.
[18] 崔丽蓉. 抗旱小麦长武 134 GAPDH基因的表达及克隆[D]. 杨凌: 西北农林科技大学, 2010.
[19] 于丽丽, 高彩球, 王玉成, 等. 柽柳甘油醛-3-磷酸脱氢酶基因的克隆与表达分析[J]. 东北林业大学学报, 2010, 38(7): 105-108.