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Acta Prataculturae Sinica ›› 2012, Vol. 21 ›› Issue (2): 116-122.

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The relevant factors of callus induction of early spring ephemeral Lachnoloma lehmannii (Brassicaceae)

MANSUR Nasir, TAN Dun-yan, LIAO Kang   

  1. Xinjiang Agricultural University, rümqi 830052, China
  • Received:2011-01-13 Online:2012-02-25 Published:2012-04-20

Abstract: Lachnoloma lehmannii is an annual early spring ephemeral Brassicaceae species in the Junggar Desert of northern Xinjiang. The primary aims of this study were to research the conditions for callus induction in L. lehmannii. Using the aspect seedling cotyledon as explants, the effect of seedling age, media, sucrose concentration and the culture condition on callus induction and their growth was investigated. The most suitable condition for callus induction was seedlings of optimal age (14 days) on an MS culture medium containing 1.0 mg/L IBA+0.1 mg/L BAP, with a suitable concentration of sucrose (3%), at a temperature of 25℃ in the dark. The best condition for callus growth was a temperature of 25℃ in the light with a photoperiod of 16 h/day.

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