Cloning and analysis of dihydroflavonol reductase (DFR) gene from Medicago sativa

Abstract

Abstract: Dihydroflavonol reductase (DFR) is a key enzyme in condensed tannin synthesis pathway. A cDNA of DFR gene is cloned from alfalfa (Medicago sativa) by using rapid amplification of cDNA ends (RACE) method. The expression pattern of MsDFR under different stresses was analyzed by qRT-PCR. The bio-informatical analysis showed that the full-length of cDNA sequence is 1 402 bp and includes a 1 023 bp open reading frame which encodes a 340-amino-acid polypeptide. A nicotinamide adenine dinucleotide phosphate (NADP) binding site “VTGASGFIGSWLVMRLMERGY” and a substrate specificity motif “TLNVTEDQKPLWDESCWSDVEFCRRV” were detected in the deduced amino acid sequence of MsDFR. The results of Real-time PCR indicate that the expression level of MsDFR gene is highest in pods, and least in root. The expression of MsDFR gene under stress of NaCl and GA₃ is down-regulated. In dark environment, the MsDFR gene expression was induced. There is a gibberellin (GA) signaling-independent pathway of tannin synthesis in alfalfa.

CLC Number:

DONG Jie, WANG Xue-min, WANG Zan, GAO Hong-wen, SUN Gui-zhi. Cloning and analysis of dihydroflavonol reductase (DFR) gene from Medicago sativa[J]. Acta Prataculturae Sinica, 2012, 21(2): 123-132.

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