Abstract: By 3-year investigation and study on wild animal resource in Sunan and Subei counties, the result indicated that the major wild terrestrial vertebrate on the grassland area in the Qilianshan Mountains and Mazongshan Mountains could be divided into 5 faunae, alpine desert animal fauna, alpine meadow animal faunal, desert animal fauna, marsh animal fauna and village-farmland animal faunal; More than 800 animal species embracing ungulate, carnivore, reptile, amphibian, fish, rodent, avifauna and insect were located in the area; Among the wild animals, 24 species were the national protective animal, accounting for 26% of total wild animal and bird species in Sunan and Subei counties. Out of the protective animals, 10 species were the first class national protective animal; 24 species were the second class national protective animal.