Abstract: Using 150 and 225 kg/hm² of P and K fertilizers as basal fertilizer in the West Orchard village of Lanzhou, field experiments were conducted to study the effects of different nitrogen fertilizer levels (0, 75, 150 and 225 kg/hm²) on the cumulated dynamics of bulb biomass and nutrition, fertilizer use efficiency (NFE) in rainfed land Lanzhou lily(Lilium davidii) in order to explore its requirement pattern for the fertilizer and provide basis for its standardization cultivation. The results showed that rational N fertilization could improve the plant’s construction, nutrition transformation and accumulation efficiency, but the steps of N, P and K absorption differed, resulting in accumulation order in K＞N＞P. The nitrogen levels only affected the stage-accumulated amounts of bulb biomass and nutrients rather than their accumulative dynamic trends. The bulb yield, N accumulation and N-fertilizer use rate (NFUR) were all increased with the amounts applied N fertilizer and get at the highest values when applied 150 kg/hm² of N fertilizer, which were (8 982.1±845.8) kg/hm², (29.123±1.767) kg/hm² and (4.97±2.16)%, respectively. On the application of N fertilizers reached to 225 kg/hm²,all the indicators decreased. When applied 75 kg/hm² of N fertilizer, the NFE was the highest and then the value great significantly decreased with amounts of N fertilizer. Considering integrated factors, the basal N-fertilizers should between 75-150 kg/hm².