Cloning, sequence analysis and prokaryotic expression of the WRKY6 of potato

Abstract

Abstract: In higher plants, there are many WRKY transcription factors in WRKY family. They are widely involved in biotic stress; abiotic stress; growth; development and metabolism regulation. Cloning WRKY6 from potato by homology cloning. Analysis show that the WRKY6 belongs to the third group of WRKY family members, zinc-finger structure is C-X₇-C-X₂₃-H-X-C. Phylogeny results show WRKY6 is the most closest to AtWRKY70 with 58% similarity, By SDS-PAGE analysis, the best expression conditions of WRKY6 are that host bacteria-Escherichia coli (DE3) is growing for 8 hours under 18℃ adding 0.2 mmol/L IPTG (Isopropyl β-D-thiogalactoside), purified protein of high specificity is obtained. This research lay a solid foundation to further study the foundation of WRKY6.

CLC Number:

LI Li-qin, HUANG Yu-bi, WANG Xi-yao. Cloning, sequence analysis and prokaryotic expression of the WRKY6 of potato[J]. Acta Prataculturae Sinica, 2011, 20(2): 177-183.

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