Abstract: Copper/zinc-superoxide dismutase (CSD) is a key enzyme involved in the plant response to abiotic stress. Its content and activity is closely related to the stress tolerance of plants. The ZjCSD gene was isolated from the transcriptome database of Zoysia japonica by homologous cloning. It encoded a protein of 152 amino acid residues. Bioinformatics analysis revealed that the protein encoded by ZjCSD gene was stable, hydrophobic, acidic, fat-soluble, and located in the cytoplasm. With typical Cu²⁺ and Zn²⁺binding sites, ZjCSD belonged to the plant SOD super family. A homology analysis based on the deduced amino sequence indicated that ZjCSD had a closer relationship with CSD from Setaria italica and Zea mays than with CSDs from other plants. The expression profiles of ZjCSD in different tissues and under different stress treatments were investigated by qRT-PCR. Transcripts of ZjCSD were detected in the root, stem, and leaf. The highest transcript levels were in the leaf. The ZjCSD mRNA levels were up-regulated by salt, drought, and cadmium stress, and down-regulated by lead stress. These results suggested that ZjCSD might play a role in drought, salt, and heavy metal stress tolerance in Z. japonica.