Whole genome identification and comparative analysis of old prokaryotic genes in Glycine max and Lotus corniculatus

doi:10.11686/cyxb2017521

Abstract

Abstract: Eukaryotes have acquired a large number of genes from prokaryotes during their evolution. The identification and analysis of these genes can provide a theoretical basis for study of the systematic evolution of higher plants. In order to further investigate the significance old prokaryotic genes during the evolution of Glycine max and Lotus corniculatus, the old prokaryotic genes in these two species’ genomes were comprehensively identified using bioinformatics methods and the functions and relations of these genes were further analyzed. Our results suggest that the number of old prokaryotic genes is higher in G. max (40.6%) than in L. corniculatus (33.9%). The largest fractions of these protein-encoding genes were found to be localized in endo-symbiotic organelles, such as mitochondria and chloroplasts.Similar distributions of the domain architecture of old prokaryotic proteins were found in both G. max and L. corniculatus genomes, revealing that they may share high collinearity and conservation during evolution.Based on Gene Ontology (GO) functional classifications, we found that the old prokaryotic proteins were mainly distributed in membrane, cell, and cell parts. In terms of molecular function, the old prokaryotic proteins mainly participated in metabolic and developmental processes in G. max, which may have been caused by human selection pressure for better agronomic productivity.In terms of biological processes, the old prokaryotic proteins mainly participated in catalytic reactions and binding reactions, indicating that they may play a role as enzymes. Using comparative analysis, our work has indicated a distinctive pattern in the evolution of old prokaryotic proteins in G. max and L. corniculatus, with the results suggesting a theoretical basis for the genomic analysis of other leguminous plants.

Key words: Glycine max, Lotus corniculatus, old prokaryotic proteins, evolution

WEI Xing-yi, LI Yu, LIU Wen-xian, JIN Xiao-yu, MIN Xue-yang, ZHANG Zheng-she, Ndayambaza Boniface, WANG Yan-rong. Whole genome identification and comparative analysis of old prokaryotic genes in Glycine max and Lotus corniculatus[J]. Acta Prataculturae Sinica, 2018, 27(11): 49-57.

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