The effect of sulfur forms on growth, physiology and secondary metabolism of Isatis indigotica at the seedling stage

doi:10.11686/cyxb2018223

Abstract

Abstract: To investigate the effect of S in different chemical forms on the growth, physiology and secondary metabolism of Isatis indigotica, an experiment with six treatments [CK (no added S), Na₂SO₄, Na₂SO₃, NaHSO₃, Na₂S and Na₂S₂O₃] was set up, and the changes in leaf fresh and dry weight, photosynthetic physiology, nitrogen and sulfur metabolism, nutritional components and content of indigo and indirubin studied, using a pot trial. It was found that the fresh and dry weight were greatest in the Na₂SO₄ treatment, with the NaHSO₃ treatment being second highest. Compared with CK, the other S treatments did not significantly promote biomass accumulation. The net photosynthetic rates and chlorophyll contents in sulfur treatments were higher than those observed in CK, and the highest observed net photosynthetic rate and chlorophyll contents occurred in the NaHSO₃ and Na₂S, respectively. Na₂S₃ and Na₂SO₄ increased the activities of nitrogen and sulfur metabolism enzymes respectively compared to other treatments, with the CK being no inhibiting obvious in their effect. The responses of nutritional components to sulfur forms differed. Soluble protein and soluble amino acid concentrations were decreased in CK plants compared to S-treated plants, with the highest soluble protein, free amino acid, and soluble sugar concentrations observed in the NaHSO₃, Na₂SO₄ and NaHSO₃ treatments, respectively. In contrast to the physiological responses, the levels of secondary metabolites indigo and indirubin were the highest in Na₂S treatment. Considering both growth stimulation and secondary metabolite accumulation, the Na₂SO₄ treatment is recommended as the most beneficial in the cultivation of I. indigotica seedlings.

Key words: sulfur form, Isatis indigotica, photosynthetic physiology, nitrogen and sulfur metabolism, nutritional components, secondary metabolites

MIAO Yu-jing, GUAN Jia-li, CAO Yi-wen, SHA Jun-tao, TANG Xiao-qing, GENG Li, WANG Lei. The effect of sulfur forms on growth, physiology and secondary metabolism of Isatis indigotica at the seedling stage[J]. Acta Prataculturae Sinica, 2019, 28(3): 101-110.

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