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Acta Prataculturae Sinica ›› 2020, Vol. 29 ›› Issue (6): 127-136.DOI: 10.11686/cyxb2019299

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Isolation and identification of an anthracnose pathogen on Vicia sativa

WANG Qiong, DUAN Ting-yu*, NAN Zhi-biao   

  1. State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2019-07-01 Revised:2019-12-09 Online:2020-06-20 Published:2020-06-20

Abstract: Anthracnose in Vicia sativa is an important fungal disease affecting leaves and stems. This research combined morphological and multiple gene phylogenetic analyses, using gene sequences of an internal transcribed spacer region, actin, chitin synthase 1 and glyceraldehyde-3-phosphate dehydrogenase to identify an anthracnose pathogen (WQ) isolated from leaves and stems of vetch plants (cv. Lanjian No.2) in Xiahe County, Gansu Province. Koch’s postulates were applied to identify pathogenicity of the strains. It was found that colonies of the fungus on potato dextrose agar (PDA) were flat, with surface dark gray-olivaceous to olivaceous-black, white margin, covered by short floccose whitish aerial mycelium, reverse gray-olivaceous to iron-gray. Conidia were hyaline, unicellular, (17.5-25.0) μm×(3.75-5.00) μm, and slightly falcate. Multiple-gene phylogenetic analysis indicated that the isolate WQ and a strain of CBS 128.57 were clustered into one clade, with a confidence level of 70%. The strains (WQ) could infect V. sativa leaves through wounds. It was confirmed that the pathogen was Colletotrichum spinaciae. This is the first report of C. spinaciae on V. sativa in China. A host range test showed that C. spinaciae exhibited high pathogenicity to Onobrychis viciifolia and Avena sativa, and moderate pathogenicity to four V. sativa cultivars, and low pathogenicity to Medicago sativa and Trifolium repens. However, Colletotrichum spinaciae had no pathogenicity to Lolium multiflorum.

Key words: Vicia sativa, anthracnose, Colletotrichum spinaciae, pathogen identification, host range